Teratoma Formation Analysis
- Catalog# ASC-6008
- Size
- Inquire
$2,900/line (Depends on the sample condition, additional fees may apply. Please inquire for details).
We accept international orders.
Histology image
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Histology image
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Histology image
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Report
All-inclusive Teratoma Formation & Analysis Service for the characterization of pluriotent human and mouse ES/iPS cells. Publication-quality images and a summary report including a list of identified tissues will be provide.
- Only 0.5 million (mouse) or 1-2 million cells (human) per injection site.
- We perform both kidney and testis injections with a >90% success rate of teratoma formation.
What you get from us: - Detailed report including protocol description and a list of identified tissues with images - CD with all images in high resolution - Tissue Blocks - H & E Slides - Snap-frozen teratoma tissue (if requested)
What we need from you: - Mouse ES/iPS: 3-6 X 10^6 cells - Human ES/iPS: 20 X 10^6 cells
What makes our service unique?
We think: A Robust Protocol is Essential for Teratoma Formation.
1. Choice of injection method
Teratomas formed after subcutaneous injection often contain undefined tissue that is difficult to
identify (A-C). In contrast, injection under the kidney or testis capsule results in characteristic
teratomas with differentiated tissues derived from all three germ layers (D-I).

2. Importance of dual injection under BOTH kidney and testis capsule
Only ~50% of human iPS cell lines form teratomas in both kidney and testis. The other lines preferentially
form teratomas in either one, but not in both organ types. Therefore, we always inject into
both kidney and testis capsule (four injection sites total).

For international customers, we accept only frozen cells, please inquire for details.
Sample Report: ASC's Teratoma Formation Analysis Reports are in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research:
- DIABETES MELLITUS, JUVENILE-ONSET INSULIN-DEPENDENT; IDDM (Page 8-12)
- HUNTINGTON DISEASE; HD (Page 8-13)
- MUSCULAR DYSTROPHY, BECKER TYPE; BMD (Page 8-12)
- SEVERE COMBINED IMMUNODEFICIENCY, AUTOSOMAL RECESSIVE, T CELL-NEGATIVE, B CELL-NEGATIVE, NK CELL-NEGATIVE, DUE TO ADENOSINE DEAMINASE DEFICIENCY (Page 8-12)
- SPINAL MUSCULAR ATROPHY I; SMA1 (Page 8-15)
References:
- F.-J. Müller, J. Goldmann, P. Löser, J. F. Loring, Cell stem cell. 6, 412-4 (2010)
- N. Maherali, K. Hochedlinger, Cell stem cell. 3, 595-605 (2008)


