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ESC-Sure™ Germline Transmission Tested Fetal Bovine Serum (FBS)
ESC-Sure™ Germline Transmission Tested Fetal Bovine Serum (FBS)

• Germline transmission tested! • Mycoplasma free • Ready to use (no...
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Teratoma Formation Analysis
Teratoma Formation Analysis

All-inclusive Teratoma Formation & Analysis Service for the characterization of...
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Teratoma Formation Analysis

$2,900/line (Depends on the sample condition, additional fees may apply. Please inquire for details).

We accept international orders.

Teratoma Formation Analysis

Histology image

  • Histology image
  • Histology image
  • Report

All-inclusive Teratoma Formation & Analysis Service for the characterization of pluriotent human and mouse ES/iPS cells. Publication-quality images and a summary report including a list of identified tissues will be provide. 

Fast turnaround time as short as four weeks.
-  Only 0.5 million (mouse) or 1-2 million cells (human) per injection site. 

-  We perform both kidney and testis injections with a >90% success rate of teratoma formation.
What you get from us:
- Detailed report including protocol description and a list of identified tissues with images
- CD with all images in high resolution
- Tissue Blocks
- H & E Slides
- Snap-frozen teratoma tissue (if requested) 
What we need from you:
- Mouse ES/iPS: 3-6 X 10^6 cells 
- Human ES/iPS: 20 X 10^6 cells  

 

What makes our service unique?

We think: A Robust Protocol is Essential for Teratoma Formation.

1. Choice of injection method

Teratomas formed after subcutaneous injection often contain undefined tissue that is difficult to

identify (A-C). In contrast, injection under the kidney or testis capsule results in characteristic

teratomas with differentiated tissues derived from all three germ layers (D-I).

 

2. Importance of dual injection under BOTH kidney and testis capsule

Only ~50% of human iPS cell lines form teratomas in both kidney and testis. The other lines preferentially

form teratomas in either one, but not in both organ types. Therefore, we always inject into

both kidney and testis capsule (four injection sites total).

 For international customers, we accept only frozen cells, please inquire for details.

Sample Report: ASC's Teratoma Formation Analysis Reports are in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research:

References:

  • F.-J. Müller, J. Goldmann, P. Löser, J. F. Loring, Cell stem cell. 6, 412-4 (2010)
  • N. Maherali, K. Hochedlinger, Cell stem cell. 3, 595-605 (2008)