Teratoma Formation Analysis

Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysis after injection of cells into an animal.

Features:

• Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
• Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6,  Human ESCs/iPSCs =  1 x 10^6
• Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.

Service Includes:

• Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
• Tissue blocks and H&E stained tissue section slides

Notes:

• Frozen sample culture Fee $500 per line
• Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.) $200 per line
• Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional $100 per line.
• Frozen tissue blocks are available with additional $100.

Technical Details:

• Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
• Injection site: Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
• Service includes: Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.

Service Requisition Form

High Teratoma Formation Rate:

----------------------------------------------------------------------------------------------------------------------

Published reports of ASC's Teratoma Formation Analysis Reports can be found in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research.

References:

1.  Zhang WY, Teratoma formation: A tool for monitoring pluripotency in stem cell research. StemBook.
2.  Valamehr B (2012) SCIENTIFIC REPORTS 2: 213
3.  K. Chen et al., Stem Cell Research USA. 9, 237–248 (2012)
4.  Telugu BPVL (2011) JOURNAL OF BIOLOGICAL CHEMISTRY 286:28948-28953
5.  Deleidi M (2011) Stem Cells 29: 1052–1063
6.  Cooper O (2012) Sci Transl Med. 4(141):141ra90
7.  Zheng Z (2012) Biomaterials 33: 5821-5831
8.  Almeida S (2012) Cell Report 2: 789-798