PCR Mycoplasma Detection Kit

Mycoplasma is a serious contaminant found commonly in cell cultures in laboratories. Cells contaminated by mycoplasma may have compromised growth, morphology, physiology and even chromosomal aberrations. Such adverse changes are likely to affect the reliability of experiments that use these contaminated cells. Routine Tests to check for mycoplasma are therefore an important and necessary quality control procedure for all cell culture labs.

ASC’s PCR Mycoplasma Detection Kit was designed to identify a conserved rRNA coding region in the mycoplasma genome, enabling it to detect majority of the mycoplasma species. It is an affordable, fast, and sensitive method for routine monitoring of mycoplasma-free cell lines. The internal control designed in the kit eliminates false negatives and provides highly reliable results. This all-inclusive PCR Mycoplasma Detection Kit can be used in conjunction with a secondary detection method such as cell culture isolation, ELISA, etc.

Key features of this kit:

  • Highly sensitive PCR method to detect mycoplasma
  • > 95% of commonly occuring mycoplasma species contaminating cell lines
  • PCR Primer Mix includes an internal control to avoid false negatives and provide reliable results
  • Straightforward, easy-to-use protocol enables routine daily testing of cell lines - just use supernatant to PCR reaction
  • Significantly short protocol time provides you with results in a couple of hours

Mycoplasma strains that are detected include but are not limited to the following strains:

Mycoplasma pneumoniae, Mycoplasma mycoides, Mycoplasma capricolum, Mycoplasma hyorhinis, Mycoplasma hyopneumoniae, Mycoplasma canis, Mycoplasma genitalium, Mycoplasma ovipneumoniae, Mycoplasma flocculare, Mycoplasma yeatsii, Mycoplasma putrefaciens, Mycoplasma synoviae, Mycoplasma hominis, Mycoplasma pirum, Mycoplasma arginini, Mycoplasma bovoculi, Mycoplasma crocodyli, Mycoplasma penetrans, Mycoplasma arthritidis, Mycoplasma salivarium, Mycoplasma cynos, Mycoplasma conjunctivae, Mycoplasma dispar, Mycoplasma canadense

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