Cas9 Expressing Cell Lines

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Cas9-expressing cell lines offer a reliable and efficient alternative to traditional CRISPR/Cas9 protocols. These cell lines have a stably integrated human codon-optimized Cas9 that is expressed at high levels for guaranteed cleavage. With fewer transfections, it provides a smoother workflow with fewer transfections and subsequently a higher cell viability. You can generate your own cell line models in a uniform genetic background (isogenic cell lines) for consistent, reproducible results that are ideal for high throughput drug screening.

Choose from the following ready-to-use Cas9 expressing cells we have developed in some popular human and murine cell line models:

  • Jurkat-Cas9 cell line
  • K562-Cas9 cell line
  • HEK293-Cas9 cell line
  • mESC (mouse embryonic cell)-Cas9 cell line

We also offer option to custom engineer a Cas9 expressing cell line model in a cell line of your interest. Inquire for details.

 

Example: Jurkat-C9m cells

Guide RNA (gRNA) validation is usually performed in K562 or HEK293 cell lines. The gRNA activity validated in these cell lines cannot always be translated in blood cells. A Jurkat cell line was engineered to stably express the S. pyogenes Cas9 nuclease. This Cas9 expressing Jurkat cell line can be used for gRNA validation by transfecting the gRNA into the cell line followed by mismatch assay.The data below indicate the validation result of the guide RNAs. The Jurkat-C9m was transfected with three oligo-synthesized guide RNAs. Genomic DNA was harvested 72 hours after transfection. All three guide RNAs are shown to be active in the mismatch assay.

We introduced case studies in our poster presentation at the Tricon Conference held in SF, CA from March 10-11, 2016. 
Support Materials

mESC-C9m cells: Material Transfer Agreement

Please download and read this contract before placing order.

References
  • POSTER, Tricon conference 2016, San Francisco, CA (March 10-11, 2016)
Ordering
Catalog ID#Product Name SizePriceQTY
$1,200.00
$1,200.00
$1,200.00
$1,200.00

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