MyEZGel™ iPSC Matrix (iPSC 3D media)
The MyEZGel™ iPSC Matrix is a powerful tool for in vitro 3D cultures of human induced pluripotent stem cell (hiPSC), with more accurate in vivo predictions for life science research and development. The MyEZGel™ kit consists of a vial of patented peptide nanofiber solution, a vial of MyEZGel™ “Kicker” trigger solution and a vial of MyEZGel™ Gro. The MyEZGel™ nanofibrils are formulated into a basic or a customer-desired cell culture medium in neutral pH. A 3D microenvironment can be formed accordingly for hiPSC growth. With MyEZGel™, cells no longer suffer acidic or chill conditions; cultured cells are easily harvested from the matrix; all operating and growth procedures can be completed at room temperature or 37°C in neutral pH.
Th MyEZGel™ iPSC 3D Matrix 3D promotes robust proliferation and better cellular viability than a 2D matrix. The matrix confers a natural environment that enables natural cellular morphology, inter-cellular interactions and physiological attributes, including gene and protein expression patterns that simulate conditions in a living organism. Our 3D-grown iPSCs may even reduce the dependency on animal models and may be a more predictive model for drug screening and discovery. The 3D matrix also uses 1/5th space and ½ the amount of media as compared to cells grown in a 2D matrix.
Key Features of MyEZGelTM iPSC Matrix:
- Serum-free and Feeder-free cell culture
- Neutral pH and room temperature culture/ 37°C: No more icing or acidic conditions
- Mimics cell microenvironment
- Cells are easily encapsulated and harvested
- Fast hydrogel formation (~30 min)
- Purified Synthetic Peptide in neutral pH
- Longer shelf life
- Supports the formation of the germ layers (teratoma formation)
Applications: Drug discovery and drug screening; disease modeling; organoid research.