ASC Advances TARGATT™ Gene-Editing Technology for Mammalian Cell Line Bioproduction Applications | ASC

7/26/19 MILPITAS, Calif.--(BUSINESS WIRE)

Applied StemCell (ASC) is excited to announce the recent publication of A System for Site-specific Integration of Transgenes in Mammalian Cells in PLOS ONE.

Mammalian cell expression systems are the most commonly used platforms for producing biotherapeutic proteins. However, development of recombinant mammalian cell lines is often hindered by the unstable and variable transgene expression associated with random integration. The recent PLOS publication describes how mammalian master cell lines were established with a landing pad engineered at the Hipp11 (H11) locus. We identified the H11 locus to be a "safe harbor" locus for gene knock-in in CHO-S cells. The landing pad placed at the H11 locus includes a pair of PhiC31 bacteriophage attP sites and genes facilitating integrase-based transgene integration.

Dr. Ruby Yanru Chen-Tsai, co-founder and CSO at ASC, and co-inventor of the TARGATT™ technology, stated: “ASC has developed an efficient strategy for site-specific integration of large genes of interest. This method enables rapid and precise insertion of a single copy gene expression cassette at defined loci in mammalian cells, resulting in homogeneous transgene expression. We have successfully applied our core technology to CHO and HEK293T mammalian cell lines for bioproduction applications. These cell lines will allow for fast and efficient bioproduction cell line generation in as fast as 3 months.”

ASC has also successfully employed the TARGATT™ technology in custom animal model generation service to allow the generation of site-specific knock-in animal models for a variety of research applications. In addition to CHO-S and HEK293T cell lines, ASC also offers products including the docking-site ready TARGATT™ “attP” mice, transgenic kits and plasmids for mouse model engineering in your own animal facilities. This technology is currently available for licensing.

For further details regarding licensing of this technology and service/ product offerings, please contact us. 

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