Humanized Strategy
Validation Data
Figure 1. Genotyping of WT, KO & Humanized mice by PCR. Primer P1/2 Wild type = 342 bp Primer P3/4 mutant = 332 bp M: 100bp Plus DNA ladder.
Figure 2. Western blot analysis of APOE3 protein from WT, KO & Humanized mice
Validate Human APOE Expression by Western Blot
KO: Apoe knockout mouse sample; hAPOE2 : humanized APOE2 mouse sample (ASHU-190013); hAPOE3 : humanized APOE3 mouse sample (ASHU-190003); hAPOE4 : humanized APOE4 mouse sample (ASHU-190002); WT: wild type mouse sample; M: marker.