Safe Harbor Locus Master iPSC Genome Editing Service
Custom “Master” iPSC Cell Line Generation using TARGATT™ technology! The TARGATT™ Master Human iPSC Line contains an attP “docking site” at the hROSA26 safe harbor genomic locus. Any gene of interest can be stably inserted at the docking site using a corresponding “attB” containing TARGATT™ donor plasmid, with guaranteed gene expression. The efficiency of this phiC31 integrase-mediated recombination is up to 100% efficiency with drug selection and up to 30% without drug selection.
Our cell line model generation experts can also engineer your safe harbor locus knock-in iPSC lines using CRISPR/Cas9 for a multi-technology approach to generate a cell line model for your specific research needs.
ASC engineered Site-Specific Knock-in of TARGATT™ sites, with mCherry reporter (left). Original Cell Line: hiPSC (right).
Safe harbor locus master cell lines are ideal for applications using reporter lines for cell tracking, for cell purification using surface markers, and for directed differentiation of iPSCs using cell-specific promoters.
iPSC lines with cell-specific promoters:
Express cell-type specific transcription factors to induce differentiation of specific cell lineages
Reporters to track cell differentiation process
iPSC lines with excisable Cas9 or nickase for efficient CRISPR gene modification
High-efficiency, high purity cells differentiated from iPSC lines
Applied StemCell can also create Site-Specific Knock-in cell lines for primary cells or other cell lines.
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