CRISPR / Cas9 Vector Design & Construction
Applied StemCell is one of the earliest licensees and provider of CRISPR/Cas9 genome editing services. We are experts in designing and cloning components for CRISPR-based gene editing in cell lines and animal models using benchmark strategies and optimized protocols. Our scientists have designed > 1000 CRISPR projects for researchers worldwide, and we use the latest sequencing and next generation sequencing (NGS) protocols to validate your gRNAs and to ensure high efficiency gene modification in your gene of interest.
We offer CRISPR/Cas9 vector design, construction and gRNA validation for:
- Cell line gene modification
- Microinjection into embryos for animal model generation
- Virus packaging
We also offer comprehensive custom CRISPR cell line and mouse/ rat model generation services.
- Hu, Q., Li, C., Wang, S., Li, Y., Wen, B., Zhang, Y., ... & Nguyen, T. K. (2019). LncRNAs-directed PTEN enzymatic switch governs epithelial–mesenchymal transition. Cell research, 1.