CHO Cells
Site-specific transgene knock-in using TARGATT CHO Master Cell Lines for the creation of high protein/antibody expression in bioproduction cells.
Bioproduction
The traditional CHO antibody production and animal bioproduction methods are inefficient because they require random gene insertion and forced amplification of transgenes. Applied StemCell has addressed these problems by utilizing our expertise in cell line and animal model engineering with our proprietary TARGATT™ technology to develop two bioproduction methods: TARGATT™ CHO Master Cell Lines and Transgenic TARGATT™ Rabbits. Both methods offer low-cost, efficiency, consistent protein expression and high protein yield that is easily scalable for large scale bioproduction.
Bioproduction Categories
CHO Cells
Transgenic Animals - Rabbit
Products and Services
Support Materials
Brochure/ Flyer
Posters:
Application Notes
CHO Bioproduction
- Safe-Harbor Locus: H11 (non-optimized locus)
- Proteins Expressed: Two therapeutic antibodies, Ab1 and Ab2
- Copies Inserted: 1 (single copy)
- Production Parameters:
- 17-day fed-batch shake-flask
- Bulk enriched pools (no single-cell cloning)
- Yield: ~1 g/l
Biopharming
- Bioproduction in rabbit milk
- TdTomato proof-of-concept
- Tdtomato Molecular weight = 56 kD
- Factor VII molecular weight = 52 kD
- F1 heterozygous rabbit (1 copy)
- Yield: 18g/l in homozygous rabbits
Technical Details
Publications
- Chi, X., Zheng, Q., Jiang, R., Chen-Tsai, R. Y., & Kong, L. J. (2019). A system for site-specific integration of transgenes in mammalian cells. PLOS ONE, 14(7), e0219842.
