ESC tested Fetal Bovine Serum (FBS)
Embryonic stem cells require very stringent protocols for growth without differentiation. ESC-Sure™ ESC-qualified Fetal Bovine Serum (FBS) is a high quality, prescreened serum that is especially suited for use in embryonic and induced pluripotent stem cell (ESC, iPSC) culture. It strengthens the growth of ESCs with normal stem cell morphology while maintaining them in their undifferentiated and pluripotent state.
Our ESC-Sure™ FBS can provide the assurance scientists need for ES-cell culturing while saving time, money and effort. A certificate of analysis will be provided with each lot of ESC-Sure™ FBS.
- Quality tested for > 5 passages of mESCs
- Minimal endotoxin levels and hemoglobin content
- Low batch to batch variation
- Serum from aseptically collected blood in USDA-approved abattoirs
Ideally, removing the bottle of serum from frozen storage and allowing to thaw overnight at room temperature (or 12 hours) is the best way. Homogenize the serum once thawed without making the serum foam. If you are not able to plan accordingly, you can also thaw the bottle of serum in a shaking/heated water bath set to 37⁰C until completely thawed.
Heat Inactivation degrades complement proteins that could interfere with specific assays. For most cell culture settings and applications, heat inactivation is not recommended. Effects of Heat Inactivation include a reduction in growth factors, increase of deposits which are commonly mistaken for microbial contamination and reduce performance of the serum.
Figure. ESC-Sure™ FBS is extensively tested for supporting undifferentiated growth of mESCs. The image shows healthy mESCs that were cultured in a MEF-feeder cell system along with Applied StemCell’s ES-grade FBS.
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For more references, visit our reference page..