Free Webinar! TARGATT™: A Mammalian Cell Library System for Antibody/ Protein Screening | ASC

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Please join Applied StemCell’s Free Webinar titled, “TARGATT™: A Mammalian Cell Library System for Antibody/ Protein Screening”

Webinar Details:

Speaker:              Ling-Jie Kong, PhD (Vice President, Research and Technology Development, Applied StemCell)

Date:                    Tuesday, October 23, 2018

Time:                    10:00 AM PST

Registration URL: https://attendee.gotowebinar.com/register/7253141052291513602?source=Web

Abstract

Mammalian cell-based libraries offer a physiologically relevant context for high throughput screening of high affinity antibodies. Current technologies using retroviruses to develop mammalian cell libraries result in random, multiple copy integration at various loci of the genome. As a result, the expression level amongst different cells in the library is varied leading to false representation of the binding affinity of individual molecules. In this talk, we introduce the TARGATT™ site-specific, integrase-mediated gene integration technology which provides an efficient strategy for integrating a gene of interest into a preselected, transcriptionally active locus. A TARGATT™-HEK293 master cell line was generated to enable knock-in of only a single copy of the transgene in every cell at the same locus, with efficiency over 90%. The TARGATT™ system thus provides an efficient platform with uniform transgene expression and reproducibility for constructing large isogenic cell libraries for rapid, high throughput antibody screening. The same system can also be used for bioproduction, protein evolution, target validation and safety assessment in immuno-oncology, GPCR and ion channels.

Key Points

  • Introduction to Mammalian cell-based libraries for screening antibodies; current protocols and limitation
  • TARGATT™ technology overview: site-specific, integrase-mediated gene integration
  • Case studies using TARGATT™ HEK293 and CHO Master Cell Lines
  • Comparison with other technologies in drug discovery, screening and bioproduction

Who should attend

Researchers who are interested in:

  • Drug discovery
  • Antibody Screening
  • Antibody discovery
  • Protein Evolution
  • Membrane-bound protein expression
  • Bioproduction
  • Immuno-oncology: CAR-T cell, novel biomarkers and checkpoints
  • Receptor identification: GPCR, ion channels

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