Transgenic Rat Model Generation Service Using BAC
- Standard
- Fully Customizable - Inquire
Standard Deliverables:
- At least 2 founders (or germline transmitted F1s) with confirmed gene knockout
- Milestone update/report; final report with a detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results
Standard Workflow and Timeline:
Service | Time |
1. DNA BAC vector design, cloning, and recombineering | 6-8weeks |
Design, strategy, and BAC recombineering | |
Enzyme digestion identification and sequencing to confirm
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|
Purification of BAC DNA for microinjection
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2. DNA pronuclear microinjection and implantation of embryos
|
2 month |
Pronuclear microinjection and embryo implantation
|
7-10 weeks |
3. Animal Care, Housing, and Genotyping Founders
|
1 month
|
Deliverable: Confirmed positive founder(s)
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For the knockout of an essential gene, ASC offers PrimeCKO™ Rat Knockout Mouse Model Generation service.
All our animals are housed in a facility that is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, has an Assurance on file with the Office of Laboratory Animal Welfare (OLAW), and is a registered research facility with the U.S. Department of Agriculture.