Onco-Ref™ FFPE Cell Scrolls

Our Onco-Ref™ FFPE Cell Scrolls are 20 µm thick sections rolled into scrolls or (rolls) and placed in a nucelic acid-free vial for quick and direct nucleic acid extraction. 

Download a copy of our ONCOREF™ information (PDF) from below:

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FFPE Scrolls ONCOREF - Reference Standards

Our ONCOREFtm FFPE Mutation Cell Scrolls are prepared from expanding our ONCOREFtm  Isogenic Cell Lines that were prepared into a FFPE Mutation Cell Blocks and finally we cut a 20µm scrolls (i.e sections or rolls).  We typically can extract > 500 ng of gDNA from each FFPE CellScroll using the Promega Maxwell Extraction system.

Our Reference Standard Features:

•  Most comprehensive MAPK mutation panel on the market
•  Footprint-free genome editing
•  Isogenically-paired mutant and wild-type cell lines
•  Homozygous knock-in of the desired mutation
•  Reference cell lines are expanded from single-cells, ensuring maximum homogeneity
•  Available in multiple formats, including slides, scrolls, and full FFPE blocks

Need FFPE cell blocks , scrolls or slides using using your own cell lines?  We offer  Custom FFPE Services, or contact us for more details.

To learn more about our FFPE Reference Standards and other diagnostic products, WATCH our WEBINAR!

Technical Details

Consistent Source of Biorelevant Specimens

- FFPE bio-mimetic specimens using FFPE cell line blocks

TECHNICAL-ONCOREF-MAPK-FFPE-cellline-blocks

Support Materials

ONCOREF™ Reference Standards: Application of CRISPR/Cas9 to the Generation of Isogenic Cell Lines and Reference Materials (October 2016)

2016-Oncoref-webinar

CRISPR/Cas9 is rapidly enabling the development of new tools for enhancing our understanding oncogenic mutations in cancer. In order to aid in advancing cancer diagnosis and treatment, Applied StemCell has recently engineered a series of 40 isogenic cell lines that feature diverse mutations in the MAPK pathway. These mutant lines are available as isogenic pairs for applications in lead compound discovery, or as FFPE and nucleic acid reference materials for assay development. This webinar will focus on ASC’s efforts in developing these research tools, as well as applications of the materials for the advancement of cancer research.

Highlights of this talk:

  • Overview of molecular reference materials 
  • Workflow and QC for ONCOREF™ cell line generation
  • Advantages of CRISPR-engineered molecular reference standards
  • Applications of reference materials in assay development
  • Q & A
FAQ

1. What is the thickness of the FFPE Isogneic cell line scrolls?

Answer: We prepare 20 µm sections using 6.5 mm diamater FFPE cell blocks.

2. How are your cell lines generated? 

Answer: ASC uses CRISPR/Cas9 technology to enable precision genome editing in its MAPK mutation panel series.  This technology allows for footprint-free gene modification, meaning that you don’t have to worry about the presence of selection markers or other genomic footprints during the development of your assay technologies.

3. How do you validate mutational status in your cell lines? 

Answer: All of our MAPK mutation panel cell lines are expanded from single clones.  This ensures maximum homogeneity of the genetic profile.  After clonal expansion, we confirm the mutational status of the cell line using Sanger sequencing.

4. Do you offer mixtures of mutant and wild-type DNA? 

Answer: We are currently working with collaborators to develop these products.  Please inquire for additional information.

References
  • Akinleye A, Furqan M, Mukhi N, Ravella P, Liu D (2013) MEK and the inhibitors: from bench to bedside. J. Hematol. Oncol. 6, 1–11
  • Santarpia L, Lippman SM, El-Naggar AK (2012) Targeting the MAPK–RAS–RAF signaling pathway in cancer therapy, Expert Opin. Ther. Targets 16, 103–119
  • Relling MV, Evans WE (2015) Pharmacogenomics in the clinic, Nature 526, 343–350
Ordering
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