Catalog Number ASE-5080; ASE-5081; ASE-5082
Quantity 2.5 x 106 viable cells/mL; 10.0 x 106 viable cells/mL; 25.0 x 106 viable cells/mL
Description Human bone marrow mononuclear cells are isolated from whole bone marrow of a single donor. This heterogeneous cell population allows researchers to isolate various cell types of the immune system. Human bone marrow mononuclear cells contain CD34+ stem cells. Human bone marrow mononuclear cells enable researchers to investigate the immune system, stem cells, and regenerative medicine and may be used for developmental studies.
These cells have been isolated from a Philadelphia-negative patient diagnosed with chronic myeloid leukemia (CML-). CML- is characterized by the excessive growth of myeloid cells in the bone marrow, which then accumulate in the blood. CML- samples have been found negative for the Philadelphia chromosome, which is the chromosomal translocation often associated with this disorder.
Development Period Postnatal
Disease State Chronic Myeloid Leukemia, Philadelphia-negative (CML-)
Quality Control All cell processing is validated under strict protocols with IRB approval. All cells are guaranteed to meet or exceed customer’s expectations when handled according to instructions.
Passage number: Frozen cells (P0)
Cryopreservation solution: 10% DMSO
Test Conditions Cell quality is determined visually after analyzing Gram and Acid Fast stainings. Cells are grown and passed to determine sterility and mycoplasma for all lots. In addition, viability is determined prior to and after freezing. All cells are confirmed to be highly pure by molecular, cellular, or protein assays with their specific markers.
Storage and Handling
Handling Instructions Cells are shipped on dry ice. Upon arrival, place into -196°C (LN2) for long term storage or subculture immediately.
Storage Conditions Store at -196°C (LN2) upon receipt until ready to culture.
Storage Solution 10% DMSO