Catalog Number ASD-0077
Quantity 1 ug
Description Applied StemCell’s total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method.1 Residual genomic DNA is removed with RNAase-free DNAase I. Applied StemCell’s total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; Northern, dot, and slot blot analyses; primer extension; poly A+ RNA selection; RNAase/S1 nuclease protection; and microarrays.
Development Period Postnatal
Disease State Normal
Absorbance (A260/A280) 1.8 – 2.1
RNA quality is determined visually by analyzing 18S and 28S ribosomal bands using non-denaturing agarose gel electrophoresis. The RNA purity level is determined by spectrophotometry to obtain the A260/A280 ratio, which must range from 1.8-2.1. Plus, the RNA functionality is assayed by RT-PCR using primers for housekeeping gene GAPDH. RNA is confirmed to be DNA-free by RT-PCR.