Catalog Number ASE-5064
Quantity > 5.0 x 105 cells/mL
Description Human skeletal muscle myoblasts are derived from whole muscle that has been dissociated into single cells and purified using differential adhesion. Human skeletal muscle myoblasts are
from a single donor. These cells enable researchers to expand and study the role of myoblasts. They are positive for desmin, and most importantly, may be differentiated into myocytes as characterized by their mature muscle cell markers.
Human skeletal muscle myoblasts may be used for various types of in vitro, in vivo, or regenerative medicine studies in normal or diseased systems. In addition, they may be used in muscle development studies.
Development Period Postnatal
Disease State Normal
Our myoblasts upregulate the expression of myosin heavy chain (MYH7), skeletal muscle actin (ACTA1) and troponin I.
Upon differentiation, our myoblasts upregulate the expression of myosin heavy chain (MYH7), skeletal muscle actin (ACTA1) and troponin I. (Left). Immunofluorescent image of cells stained with troponin I antibody (green) and DAPI (blue). (Center) Myosin heavy chain immunofluorescent staining. (Right) RT-PCR analyses of cells collected at different time points after the start of the differentiation. Note that ACTA1 is present in confluent myoblasts (day 0), but its levels are upregulated as the differentiation progresses2.
Quality Control All cell processing is validated under strict protocols with IRB approval. All cells are guaranteed to meet or exceed customer’s expectations when handled according to instructions.
Passage number: Frozen cells (P3-4)
Cryopreservation solution: 10% DMSO
Test Conditions Cells are grown and passed to determine sterility and mycoplasma for all lots. In addition, viability is determined prior to and after freezing. All cells are confirmed to be highly pure by molecular, cellular, or protein assays with their specific markers.
Storage and Handling
Handling Instructions Frozen cells are shipped on dry ice. Upon arrival, place into -196°C (LN2) for long term storage or subculture immediately.
Storage Conditions Store at -196°C (LN2) upon receipt until ready to culture.
Storage Solution 10% DMSO