Catalog Number ASD-0043
Quantity 10 ug
Description Applied StemCell’s total RNA passes an extensive testing to ensure a high degree of purity and intactness. RNA is isolated using a modified guanidinium thiocyanate method.1 Residual genomic DNA is removed with RNAase-free DNAase I. Applied StemCell’s total RNA can be used in downstream applications such as RT-PCR and real-time RT-PCR; differential display; cDNA synthesis; Northern, dot, and slot blot analyses; primer extension; poly A+ RNA selection; RNAase/S1 nuclease protection; and microarrays.
This RNA comes from the fibroblasts of an individual with Legg-Calve-Perthes syndrome (LCP), a degenerative disease of the hip joint caused by a reduction in blood flow to the joint, which leads to some degree of collapse to the hip joint and deformity in the ball of the femur. The cause of Legg-Calve-Perthes syndrome is unknown.
Origin Human, single donor
Development Period Postnatal
Disease State Legg-Calve-Perthes Syndrome (LCP)
Absorbance (A260/A280) 1.8 – 2.1
RNA quality is determined visually by analyzing 18S and 28S ribosomal bands using non-denaturing agarose gel electrophoresis. The RNA purity level is determined by spectrophotometry to obtain the A260/A280 ratio, which must range from 1.8-2.1. Plus, the RNA functionality is assayed by RT-PCR using primers for housekeeping gene GAPDH. RNA is confirmed to be DNA-free by RT-PCR.
Storage and Handling
Handling Instructions RNA products are sensitive to degradation by temperature changes or exogenous ribonucleases that result during the handling processes. Must wear gloves at all times when handling RNA. All reagents used, tubes, and barrier pipettes must be RNAase-free.
For thawing RNA: place tube in ice and thaw to completion. Vortex tube for a few seconds and spin down RNA quickly and place in ice once again. To minimize freeze-thaw cycles (< 3), aliquot RNA as desired.
Storage Conditions Store total RNA at -80°C. Can be stored at -20°C for short term in a frost-free freezer. Avoid multiple freeze-thaw cycles. To prevent any possible RNAase contamination, always wear gloves when handling RNA. For maximum recovery of product, centrifuge the original vial after thawing and before removing the cap.
Storage Solution RNAase-free water