Neural Stem Cells (iPSC from Blood Cells)

Applied StemCell's Neural Stem Cells (NSCs) are cryo-preserved, ready-to-use cells derived from a footprint-free, karyotype normal human iPSC line. NSCs were derived using EB formation method and isolation of neural rosettes. Obtained neuroepithelial cells represent a high purity NSC population, in which >95% of cells express key NSC markers, SOX1 and Nestin. Applied StemCell's NSC lines in combination with our optimized maintenance media allows customers to obtain cells characterized by high recovery rate, and can be expanded for up to 5 passages without loss of their differentiation capacity. The NSCs have a great capacity to differentiate into various types of neurons and glial cells.

Quantity

• ≥1.0x10^6 viable cells/vial

Quality Control:

• NSC purity: ≥90% SOX1+/Nestin+
• Recovery of frozen cells: 80% viability

Figure 1. Images of Applied StemCell’s neural stem cells (NSC) taken 24 hours post-thaw without previous medium change, at 10x (left) and 20x (right) magnification.

Figure 2. Example of NSC immunostaining showing that ≥90% of total cells expressed SOX1 (green) and Nestin (red) markers. Total count of nuclei (blue) is used as the total number of cells.