Products

Applied StemCell Products

Applied StemCell Inc. (ASC) offers a large array of products for site specific transgenics, cell culture, and stem cell research. Our products encompass a wide spectrum, from: high quality transgenic kits for knock-in mice and knock-out mice generation, mouse embryonic fibroblast (MEF cells), feeder cells, growth factors, options for stem cell characterization and stem cell differentiation, as well an array of iPSC cells. See below for our full product list.

  • Genome Editing Products for Cell Line Generation

    • CRISPR Point Mutation, Knockin, Knockout Kit

      CRISPRCLEAR™ Custom Transfection-Ready Kit contains next generation sequencing validated gRNA plasmid(s) designed to target a gene in your cell line of interest, donor DNAs and Cas9 expression plasmids. The Cas9 plasmids contain wild type Cas9. For researchers who prefer a more stringent off-target profile, we also provide Cas9 D10A or H840A nickase plasmids. Each kit is custom made to specifically target your gene-of-interest. 

    • CRISPR Hematopoietic Cells Gene Editing Kit (Knock-in, Knockout, Point Mutation)

      The CRISPRCLEAR™ Kits for editing blood-derived cells is an affordable, straightforward, “do-it-yourself” tool that helps you to genetically modify blood-derived cell lines in your own lab with high efficiency. The kits are designed for generating knock-in, knockout and point mutation models in blood-derived cell lines such as Jurkat, TF-1, K562 and other lymphocytes derived from NK cells or B cells. 

    • CRISPR Safe Harbor Locus Knock-in Kit

      CRISPRCLEAR™ Safe Harbor Locus Knock-in Kit for Human Cell Lines is a CRISPR genome editing kit containing surveyor assay validated gRNA plasmid(s) designed to target the hAAVS1, hH11 or hRosa26 locus, donor DNA and Cas9 expression plasmids. This kit can be used to generate CRISPR knock-in cell lines. CRISPRCLEAR™ Safe Harbor Locus ROSA26 Knock-in Kit is also available for Mouse Cell Lines.

    • TARGATT™ Safe Harbor Locus Knock-in Kit (Mouse)

      Rapid generation of site-directed knock-in mouse ES cell lines.

      Applied StemCell, Inc., offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Generation Kit. The kit includes a mouse ES cell line of C57BL/6 strain background with a TARGATT™ docking site (attP) at a transcriptionally active locus (the H11 locus on chromosome 11), and vectors for TARGATT™ integrase and DNA integration. With this method, large DNA fragments can be inserted at the H11 locus at high efficiency with robust transgene expression.

  • Isogenic Cell Lines

  • Reference Standards

    • MAPK, KRAS, BRAF Genomic DNA (gDNA)

      Applied StemCell Inc’s MAPK genomic DNA reference standards represent biologically-relevant controls that can be directly incorporated into your sample processing workflows in order to optimize your protocols, evaluate assay sensitivity and specificity, and analyze the impact of workflow changes on downstream analysis.  They represent ideal materials for both assay development and routine monitoring of assay performance.

    • MAPK, KRAS, BRAF FFPE Reference Standards

      Cell line-based FFPE reference standards represent ideal materials for companion diagnostics assay development and routine quality control.  ASC Dx provides precision-edited, isogenically-paired, mutant and wild-type FFPE reference standards that can help streamline your assay development or quality control processes.  Our MAPK reference standards feature 51 mutations in the MAPK signaling pathway.  We utilize footprint-free genome editing for cell line generation, thereby ensuring that you get the precise mutation that you need, without having to worry about the incorporation of genomic scars from the use of selection markers or other footprint-based technologies.

    • Cancer FFPE Cell Slides

      FFPE Cancer Cell Line Slides

      ASC Dx offers slide-mounted FFPE tissue sections from 34 common cancer cell lines, derived from 10 different tissues of origin.  Our FFPE cell pellets are prepared using a patent-pending process that generates highly homogenous FFPE blocks with any cell density (up to 90% area coverage). Both morphology and biological molecules, including protein and nucleic acid (DNA and RNA) targets are well preserved for IHC and ISH analysis.

    • Mouse FFPE Cell Slide

      Mouse FFPE Cell Pellet Slide.

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    • Custom FFPE Slides

      FFPE cell pellets are commonly used as controls in immunohistochemistry (IHC) and in situ hybridization (ISH) applications. Our FFPE cell pellets are prepared using a patent-pending process that generates highly homogenous FFPE blocks with any cell density (up to 90% area coverage). Both morphology and biological molecules, including protein and nucleic acid (DNA and RNA) targets are well preserved for IHC and ISH analysis.

      •    Fixation: 10% formaldehyde/1xPBS for 24 hours at 24-26°C
      •    Section thickness: 5µm
      •    Cell Density:  >75%
      •    Slide Type: SuperFrost Plus

    • Multiplex PCR Reference Standards

      Multiplexed PCR reference standards allow you to simultaneously validate your assay performance across multiple loci and genetic alterations for PCR or next-generation sequencing (NGS). 

  • Stem Cell Research

    • MEF Feeder Cells

      Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research. We offer multiple available lines including: CF-1, DR4, Neo-resistant and SNL (STO) feeder cells.

    • ESC tested Fetal Bovine Serum (FBS)

      ESC-Sure™ ESC-qualified Fetal Bovine Serum (FBS) has been extensively tested for use in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture. It supports the growth of mouse ESCs and iPSCs and promotes pluripotency.

    • iPSC Cells, ESCs, IPS Cell Culture

      iPSC Cells (induced pluripotent stem cells) as well as mouse embryonic stem cells.

    • Neural Stem Cells (NSCs)

      Applied StemCell’s ready-to-use Neural Stem Cells (NSCs) were derived from human iPSCs under xeno-free conditions using non-integrating episomal-based reprogramming methods. These fully characterized NSCs at low passage (p1) can be further differentiated to the particular neuronal cell type of the investigator’s choice, thus facilitating studies in cell replacement therapies and neuronal disease modeling

    • iPSC Generation

      Human EZ- iPSC Generation kits - Reprogram your somatic cells to iPSC with the Retroviral or the Episomal kit.

    • Stem Cell Markers

      Stem Cell Marker kits. Characterization of embryonic stem cells and induced pluripotent stem cells by pluripotency protein or RNA marker kits and stem cell gene arrays.

    • CRISPR Kit for iPSCs

      CRISPR Genome Editing Kit (Knockout, Knock-in, Point Mutation) for your iPSCs. 

    • Stem Cell Differentiation

      Applied StemCell Inc. (ASC) developed multiple solutions for the differentiation of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC).

      We offer kits for the directed differentiation of ESCs and iPSCs into:

      • Neural Stem Cells (Neural Progenitor Cells, Motor Neurons)
      • Cardiomyocytes
      • Dendritic Cells
      • Endothelial Cells
    • Cytokines

      Cytokines

  • Primary Cells from Patient Samples

  • Xenograft Model Research Tools

    • Substrate-free Autobioluminescent Cell Lines and Vectors

      Eliminate time consuming substrate injection with Luciferase. We can quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging. 4 major cell lines (HCT116, HEK293, MCF7, T47D) engineered to be autonomously bioluminescent. Custom Cell Line Developement: we can also quickly modify your cell lines to express an autobioluminescent reporter and return them to your laboratory for in vivo imaging as well. 

       

    • MyEZ Gel™ CDX/PDX Models Injection Media

      MyEZGel CDX/PDX is a protein based solubilized base membrane synthesized from commonly used amino acids, and minerals.  The amino acids include Arginine, Aspartate, Glycine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, and Valine. MyEZGel CDX/PDX is compatible with various adhesion proteins and cell growth factors, including but not limited to laminin, collagens, VE-growth factor, fibroblast growth factor, and other growth factors. MyEZGel CDX/PDX is a powerful reagent to package and deliver cancer cells, primary cancer cells, or stem cells in vivo for xenograft animal models.

  • TARGATT™ Products for Site-Specific Knock-in Mouse Generation

    • TARGATT™ attP Mice from Charles River

      This transgenic mouse line contains an attP site at the H11 loci for easy site-specific gene knock-in and is available for purchase from Charles River. Make your own TARGATT™ knock-in mouse for gene overexpression, reporter gene insertion, knock-down by siRNA, humanized models and other applications. TARGATT™ attP Mice are available in the FVB and C57BL6 backgrounds.

                

    • TARGATT™ Embryos/ Mice

      Our TARGATT™ Embryos can be used to establish your own TARGATT™ Transgenic Mouse colony via embryo transfer. We offer high quality H11 (Hipp11) and ROSA26 TARGATT™ Embryos (Frozen) which contain an attP site in either H11 or ROSA26 locus. Both loci are available in two backgrounds, FVB and C57BL6. After setting up your TARGATT™ Transgenic Mouse colony, you can generate site-specific knock-in mice using our TARGATT™ Transgenic Kit.

    • TARGATT™ Transgenic Kits

      TARGATT™ Transgenic Kits are essential for the generation of TARGATT™ knock-in mice. The kits contain PhiC31 integrase mRNA, control mRNA and buffer and are designed for the microinjection of a TARGATT™ plasmid into the pronuclei of TARGATT™ Transgenic Mouse embryos.

    • TARGATT™ Plasmids

      We offer a wide range of plasmids for our TARGATT™ Transgenic Knock-in Mouse applications. Our TARGATT™ plasmids are engineered to contain various promoter and marker gene combination to best meet your needs when generating your transgenic mouse.

    • TARGATT™ Genotyping Kit

      The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of TARGATT™ Transgenic Mice and knock-in mice generated from TARGATT™ Embryos via tail biopsy. Use this kit to confirm the presence of an attP knock-in site (H11 or ROSA26) and/or the integration of a transgene into the TARGATT™ Site.

  • CRISPR Kits for Animal Model generation

    • Custom Mouse Injection-Ready Kit (Validated)

      The CRISPRCRITTERS™ Injection-Ready Kit is a CRISPR genome editing kit for the generation of transgenic mice and rats based on the CRISPR technology. Our CRISPR kits contain validated gRNAs designed to target your gene of interest, donor DNAs and microinjection-validated Cas9 mRNA. CRISPR kits can be used for a large variety of genome editing projects - from the introduction of point mutations to gene knockouts and knockins. With CRISPRCRITTERS™ you can generate transgenic mice at a fraction of the costs and time of traditional transgenic tools.

    • in vivo validated Cas9 mRNAs

      Applied StemCell’s Cas9 mRNA and Cas9-D10A nickase mRNA are transcribed from mammalian codon-optimized cDNA plasmid. They are part of our CRISPRCRITTERS™ kit system - a genome editing kit based on the CRISPR technology. Cas9 and Cas9-D10A nickase cut genomic DNA in combination with target-specific guide RNA (gRNA).
    • CRISPRCRITTERS™ Custom Rat Injection-Ready Kit (Validated)

      Our CRISPRCRITTERS™ Injection-Ready Kit for transgenic rat is a CRISPR kit for transgenic rat model generation. This CRISPR kit contains Surveyor Assay validated gRNAs designed to target your gene of interest in rats, donor DNAs and microinjection-validated Cas9 mRNA. For customers who prefer a more stringent off-target profile, we also provide mRNAs of Cas9 D10A or H840A nickase.