Animal Model Generation
In vivo-grade, injection-ready CRISPR kits for the generation of transgenic mice and rats.
The CRISPRCRITTERS™ Injection-Ready Kit is a CRISPR genome editing kit for the generation of transgenic mice and rats based on the CRISPR technology. Our CRISPR kits contain in vivo validated gRNAs designed to target your gene of interest, donor DNAs and microinjection-validated Cas9 elements. CRISPR kits can be used for a large variety of genome editing projects - from the introduction of point mutations to gene knockout and knock-in. With CRISPRCRITTERS™ you can generate transgenic mice at a fraction of the costs and time of traditional transgenic tools.
Our CRISPRCRITTERS™ Injection-Ready Kit for transgenic rat is a CRISPR kit for transgenic rat model generation. This CRISPR kit contains Surveyor Assay validated gRNAs designed to target your gene of interest in rats, donor DNAs and microinjection-validated Cas9 mRNA. For customers who prefer a more stringent off-target profile, we also provide mRNAs of Cas9 D10A or H840A nickase.
Applied StemCell’s Cas9 mRNA and Cas9-D10A nickase mRNA are transcribed from mammalian codon-optimized cDNA plasmid. They are part of our CRISPRCRITTERS™ kit system - a genome editing kit based on the CRISPR technology. Cas9 and Cas9-D10A nickase cut genomic DNA in combination with target-specific guide RNA (gRNA).
We are experts in CRISPR Service! Applied StemCell is in Nature Biotechnology as one of the select companies for CRISPR-Cas9 tools! Monya Baker, "Gene Editing at CRISPR Speed," Nature Biotechnology, 32: 309-312, April 2014.