CRISPRCRITTERS™ Custom Rat Injection-Ready Kit (Validated)
CRISPRCRITTERS™ Injection-Ready Kit is a CRISPR kit for the generation of transgenic mice and rats. This CRISPR rat model kit contains Surveyor Assay validated gRNAs designed to target your gene of interest, donor DNAs and microinjection-validated Cas9 mRNA. For customers who prefer a more stringent off-target profile, we also provide mRNAs of Cas9 D10A or H840A nickase.
- Injection Ready
- Validated in Surveyor Assay
- Cas9 Nickase Available
- Full Rat Generation Service Available
Injection-Ready Validated CRISPR Kits for Rat Model Generation
1. Do you provide validated gRNAs in the CRISPRCRITTERS™ kit?
Yes, the gRNAs included in the mouse kits are validated in vivo in mouse embryos, and by in vitro validation in cultured rat cells for rat CRISPRCRITTER™ Kits.
2. How do you calculate indel rate?
We use the formula: indel rate = # blastocysts with indels/ # total blastocysts tested.
3. What are the contents of the CRISPRCRITTERS™ Kits?
The kit contains individually aliquoted reagents sufficient for 3 rounds of microinjection: (1) in vivo validated total sgRNA (1 µg x 3); (2) total donor ssODN (5 µg x 3) or donor plasmids (0.25 µg x 3); (3) optimized Cas9 protein (2.5 µg x 3). We do not recommend freeze-thawing of reagents after the 1st use for microinjections.
4. Do you also validate gRNAs in CRISPCRITTERS™ Kits for Rats using in vivo validation in rat embryos?
Due to limitations in culturing rat embryos, we include only in vitro validated gRNAs in the rat kits.
5. What are the criteria to be considered for designing a conditional knockout (CKO) CRISPRCRITTERS™ Kit?
We use two criteria for designing the CKO CRISPRCRITTERS™ Kits: (1) Retain the 1st exon if possible and target the remaining early exons (provided the length of the targeted exon(s) is not a multiple of 3) for inserting flanking LoxP sites; (2) The distance between the two LoxP sites should be > 6kb.
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