Cas9 Expressing Cell Lines
Cas9-expressing cell lines offer a reliable and efficient alternative to traditional CRISPR/Cas9 protocols. These cell lines have a stably integrated human codon-optimized Cas9 that is expressed at high levels for guaranteed cleavage. It provides a smoother workflow with fewer transfections and subsequently a higher cell viability. You can generate your own knockout, gene knock-in and gene tagging cell line models in a uniform (isogenic) genetic background for consistent, reproducible results that are ideal for antibody validation, high throughput drug screening, and gRNA validation.
Choose from the following ready-to-use Cas9 expressing cells we have developed in some popular human and murine cell line models:
- Jurkat-Cas9 cell line
- K562-Cas9 cell line
- HEK293-Cas9 cell line
- mESC (mouse embryonic cell)-Cas9 cell line
Example: Jurkat-C9m cells
Guide RNA (gRNA) validation is usually performed in K562 or HEK293 cell lines. The gRNA activity validated in these cell lines cannot always be translated in blood cells. A Jurkat cell line was engineered to stably express the S. pyogenes Cas9 nuclease. This Cas9 expressing Jurkat cell line can be used for gRNA validation by transfecting the gRNA into the cell line followed by mismatch assay.The data below indicate the validation result of the guide RNAs. The Jurkat-C9m was transfected with three oligo-synthesized guide RNAs. Genomic DNA was harvested 72 hours after transfection. All three guide RNAs are shown to be active in the mismatch assay.
We introduced case studies in our poster presentation at the Tricon Conference held in SF, CA from March 10-11, 2016.
mESC-C9m cells: Material Transfer Agreement
Please download and read this contract before placing order.
- POSTER, Tricon conference 2016, San Francisco, CA (March 10-11, 2016)