CRISPR Cell Lines (Cas9 Expressing Cell Lines) Jurkat, mESC, K562
Cas9 expressing Cell Lines (Jurkat, mESC, K562)
Guide RNA (gRNA) validation is usually performed in K562 or HEK293 cell lines. The gRNA activity validated in these cell lines cannot always be translated in blood cells. A Jurkat cell line was engineered to stably express the S. pyogenes Cas9 nuclease. This Cas9 expressing Jurkat cell line can be used for gRNA validation by transfecting the gRNA into the cell line followed by mismatch assay.
The data below indicate the validation result of the guide RNAs. The Jurkat-C9m was transfected with three oligo-synthesized guide RNAs. Genomic DNA was harvested 72 hours after transfection. All three guide RNAs are shown to be active in the mismatch assay.
We recently introduced case studies in our poster presentation at the Tricon Conference held in SF, CA from March 10-11, 2016.
- POSTER, Tricon conference 2016, San Francisco, CA (March 10-11, 2016)