Genome Editing Products for Cell Line Generation
Applied StemCell offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Generation Kits and CRISPR Genome Editing Kits.
Genome Editing Technologies Comparison:
CRISPRCLEAR™ Custom Transfection-Ready Kit contains next generation sequencing validated gRNA plasmid(s) designed to target a gene in your cell line of interest, donor DNAs and Cas9 expression plasmids. The Cas9 plasmids contain wild type Cas9. For researchers who prefer a more stringent off-target profile, we also provide Cas9 D10A or H840A nickase plasmids. Each kit is custom made to specifically target your gene-of-interest.
The CRISPRCLEAR™ Kits for editing blood-derived cells is an affordable, straightforward, “do-it-yourself” tool that helps you to genetically modify blood-derived cell lines in your own lab with high efficiency. The kits are designed for generating knock-in, knockout and point mutation models in blood-derived cell lines such as Jurkat, TF-1, K562 and other lymphocytes derived from NK cells or B cells.
CRISPRCLEAR™ Safe Harbor Locus Knock-in Kit for Human Cell Lines is a CRISPR genome editing kit containing surveyor assay validated gRNA plasmid(s) designed to target the hAAVS1, hH11 or hRosa26 locus, donor DNA and Cas9 expression plasmids. This kit can be used to generate CRISPR knock-in cell lines. CRISPRCLEAR™ Safe Harbor Locus ROSA26 Knock-in Kit is also available for Mouse Cell Lines.
Rapid generation of site-directed knock-in mouse ES cell lines.
Applied StemCell, Inc., offers a large selection of gene-targeting products including TARGATT™ Site-specific Knock-in Cell Generation Kit. The kit includes a mouse ES cell line of C57BL/6 strain background with a TARGATT™ docking site (attP) at a transcriptionally active locus (the H11 locus on chromosome 11), and vectors for TARGATT™ integrase and DNA integration. With this method, large DNA fragments can be inserted at the H11 locus at high efficiency with robust transgene expression.