Stem Cell Research
Applied StemCell offers multiple products for embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) generation, stem cell characterization and stem cell differentiation. See below for our reagents and kits for the generation of iPS cell lines, characterization of pluripotent stem cells, and differentiation of pluripotent stem cells. We also provide individual components for our stem cell generation, stem cell characterization and stem cell differentiation kits.
Applied StemCell’s ready-to-use Neural Stem Cells (NSCs) were derived from human iPSCs using non-integrating episomal-based differentiation methods. These fully characterized NSCs at low passages and can be further differentiated to the particular neuronal cell type of the investigator’s choice, thus facilitating studies in cell replacement therapies and neuronal disease modeling.
Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research. We offer multiple available lines including: CF-1, DR4, Neo-resistant and SNL (STO) feeder cells. All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.
- Diesease specific KO lines and isogenic controls
- Knock-in Reporters
iPSC Cells (induced pluripotent stem cells) as well as mouse embryonic stem cells.
iPSC/ ESC Cell lines: Applied StemCell’s iPSC cell line catalog includes footprint-free human iPSCs (hiPSCs) reprogrammed from fibroblasts, adipocytes and PBMCs from healthy and diseased patients. These hiPSCs are fully characterized for pluripotency and can be readily used for ips cell culture applications and for differentiation into cellular lineages of choice.
The ASC stem cell catalog also includes iPSCs derived from mouse, pig and guinea pigs using episomal or retroviral reprogramming methods, and various mouse ESC lines that have been tested for successful germline transmission.
ESC/ iPSC Cell Culture Products: Applied StemCell also supports its iPSC pipeline with medium and supplements for optimal and robust iPSC cell culture. Our catalog includes media for culturing human and mouse iPSC/ ESCs, including ESC- qualified FBS and serum/ feeder-free (SFFM) medium.
iPSC 3D media, iPSC Culture Media
ESC-Sure™ ESC-qualified Fetal Bovine Serum (FBS) has been extensively tested for use in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture. It supports the growth of mouse ESCs and iPSCs and promotes pluripotency.
Human EZ- iPSC Generation kits - Reprogram your somatic cells to iPSC with the Retroviral or the Episomal kit.
Stem Cell Marker kits. Characterization of embryonic stem cells and induced pluripotent stem cells by pluripotency protein or RNA marker kits and stem cell gene arrays.
Applied StemCell Inc. (ASC) developed multiple solutions for the differentiation of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC).
We offer kits for the directed differentiation of ESCs and iPSCs into:
- Neural Stem Cells (Neural Progenitor Cells, Motor Neurons)
- Dendritic Cells
- Endothelial Cells