iPSCs (Master iPSCs for Neural Differentiation)
Applied StemCell has several “master” control iPSC-cell lines (feeder-free, integration-free) from multiple donors and tissue sources that are suitable for CRISPR/Cas9 genome editing, to differentiate to isogenic somatic cell lineages (neural stem cells, astrocytes, neurons, and more), and for drug toxicity and efficacy screening applications.
Also available are isogenic panels of neural cell derivatives from these master iPSCs (neural stem cells, dopaminergic neurons and mixed neurons, and astrocytes, that are ideal for comparative neuroscience studies, drug and toxicity screening. Our catalog of iPSC products also includes differentiation and maintenance media and kits, to derive your own terminally differentiated cell lines from our iPSCs.
Differentiation of ASE-9109 control iPSC line into neural lineage cells
Figure 1. Neural lineage differentiation of control iPSC line ASE-9109 derived from male cord blood cells. Neural stem cells: Nestin/Sox1/DAPI; Mixed neurons: Tuj1 or GABA/ MAP2/ DAPI; Astrocytes: GFAP/DAPI.
Dopaminergic neurons differentiated from control iPSC line ASE-9109
Figure 2. Dopaminergic neurons differentiated from male cord blood control iPSC line ASE-9109. Immunocytochemical analysis of neuronal marker, Tuj1 (neuronal type β III tubulin; green); dopamine neuron marker, TH (tyrosine hydroxylase; red); and nuclear marker, DAPI (blue).
These iPSC lines were derived from CD34-positive (CD34+) human cord blood cells using "integration-free" episomal reprogramming methods in feeder-free conditions.
Applied StemCell has several control iPSC-cell lines from multiple donors that are fully characterized for pluripotency, normal karyotype, and teratoma formation. These iPSCs have been derived using feeder-free, non-integrating reprogramming methods. The cell lines have also been tested and used extensively for generating CRISPR-Cas9-engineered iPSC-lines for disease modeling and drug screening purposes.
Also available are isogenic panels of neuronal derivatives from these iPSCs (neural stem cells, dopaminergic neurons and mixed neurons, and astrocytes, that are ideal for comparative neuroscience studies, drug and toxicity screening. Our catalog of iPSC products also includes differentiation and maintenance media and kits, to derive your own terminally differentiated cell lines from our iPSCs.
Advantages in choosing ASC's control iPSC lines:
- Fully characterized stem cell lines: expression of pluripotency markers, normal karyotype, and teratoma formation into three germ layers
- Suitable for genetic modification using CRISPR-Cas9, and as isogenic control for engineered cell lines
- Easy differentiation protocols to generate isogenic panels of neuronal lineage cells (dopaminergic and motor neurons, astrocytes and oligodendrocytes)
- iPSCs from multiple donors and tissue resources provide a broad genetic background for basic research, drug and toxicity screening application
Control-iPSC cell lines include:
- ASE-9209: iPSC-derived from normal human fibroblasts (Female)
- ASE-9109: iPSCs-derived from normal human cord blood (Male)
- ASE-9110: iPSCs-derived from normal human cord blood (Female)
Master iPSC Characterization
Figure 1. Characterization data for Male cord blood cells derived iPSC line ASE-9109. Immunocytochemical staining for pluripotency markers NANOG, OCT4, TRA-1-60, and TRA-1-81.
Figure 2. Karyotype analysis for control iPSC line ASE-9109. Normal karyotype results for ASE-9109 control iPSC line derived from male cord blood CD34+ cells.
Figure 3. Characterization of control iPSC line ASE-9110 derived from female CD34+ cord blood cells. Immunocytochemical staining for pluripotency markers SOX2, NANOG, OCT4, TRA-1-60, and TRA-1-81.
We also have Custom iPSC Generation and Differentiation Services are also available. Please inquire
Control iPSC Lines:
- Tanaka, H., Homma, H., Fujita, K., Kondo, K., Yamada, S., Jin, X., ... & Atsuta, N. (2020). YAP-dependent necrosis occurs in early stages of Alzheimer’s disease and regulates mouse model pathology. Nature Communications, 11(1), 1-22.
- Su, S., Guntur, A. R., Nguyen, D. C., Fakory, S. S., Doucette, C. C., Leech, C., ... & Sims-Lucas, S. (2018). A renewable source of human beige adipocytes for development of therapies to treat metabolic syndrome. Cell reports, 25(11), 3215-3228.
- Lizarraga, S. B., Maguire, A. M., Ma, L., van Dyck, L. I., Wu, Q., Nagda, D., ... & Cowen, M. H. (2018). Human neurons from Christianson syndrome iPSCs reveal allele-specific responses to rescue strategies. bioRxiv, 444232.
- Tanaka, H., Kondo, K., Chen, X., Homma, H., Tagawa, K., Kerever, A., ... & Fujita, K. (2018). The intellectual disability gene PQBP1 rescues Alzheimer’s disease pathology. Molecular Psychiatry, 1.
- Kavyasudha C., Macrin D., ArulJothi K.N., Joseph J.P., Harishankar M.K., Devi A. (2018) Clinical Applications of Induced Pluripotent Stem Cells – Stato Attuale. In: Advances in Experimental Medicine and Biology. Springer, New York, NY. https://doi.org/10.1007/5584_2018_173.
- Lin, Y., Linask, K. L., Mallon, B., Johnson, K., Klein, M., Beers, J., ... & Zou, J. (2017). Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin‐Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem cells translational medicine, 6(2), 527-538.
iPSC-differentiated cell lines
- Gupta, G., Gliga, A., Hedberg, J., Serra, A., Greco, D., Odnevall Wallinder, I., & Fadeel, B. Cobalt nanoparticles trigger ferroptosis‐like cell death (oxytosis) in neuronal cells: Potential implications for neurodegenerative disease. The FASEB Journal.
- Kussauer, S., David, R., & Lemcke, H. (2019). hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro-Electrode-Array Analyses Can Tell Us. Cells, 8(11), 1331.
- Cheng, F., Fransson, L. Å., & Mani, K. (2019). The cyanobacterial neurotoxin β-N-methylamino-l-alanine prevents addition of heparan sulfate to glypican-1 and increases processing of amyloid precursor protein in dividing neuronal cells. Experimental Cell Research. https://doi.org/10.1016/j.yexcr.2019.03.041
- Daily, N. J., et al. (2017). High-Throughput Phenotyping of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes and Neurons Using Electric Field Stimulation and High-Speed Fluorescence Imaging. ASSAY and Drug Development Technologies. 15(4): 178-188. https://doi.org/10.1089/adt.2017.781
- Daily, N. J., Santos, R., Vecchi, J., Kemanli, P., & Wakatsuki, T. (2017). Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools. Journal of evolving stem cell research, 1(2), 1.
- Daily, N. J., et al. (2015). Journal of Bioengineering & Biomedical Science, 2015.
- Shaltouki, A., Sivapatham, R., Pei, Y., Gerencser, A. A., Momčilović, O., Rao, M. S., & Zeng, X. (2015). Mitochondrial alterations by PARKIN in dopaminergic neurons using PARK2 patient-specific and PARK2 knockout isogenic iPSC lines. Stem cell reports, 4(5), 847-859.
- Efthymiou, A. G., Steiner, J., Pavan, W. J., Wincovitch, S., Larson, D. M., Porter, F. D., ... & Malik, N. (2015). Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling. Stem cells translational medicine, 4(3), 230-238.
- Efthymiou, A., Shaltouki, A., Steiner, J. P., Jha, B., Heman-Ackah, S. M., Swistowski, A., ... & Malik, N. (2014). Functional screening assays with neurons generated from pluripotent stem cell–derived neural stem cells. Journal of biomolecular screening, 19(1), 32-43.
- Shaltouki, A., Peng, J., Liu, Q., Rao, M. S., & Zeng, X. (2013). Efficient generation of astrocytes from human pluripotent stem cells in defined conditions. Stem cells, 31(5), 941-952.