iPSCs (Master iPSCs for Neural Differentiation)
Applied StemCell has several control iPSC-cell lines from multiple donors that are fully characterized for pluripotency, normal karyotype, and teratoma formation. These iPSCs have been derived using feeder-free, non-integrating reprogramming methods. The cell lines have also been tested and used extensively for generating CRISPR-Cas9-engineered iPSC-lines for disease modeling and drug screening purposes.
Also available are isogenic panels of neuronal derivatives from these iPSCs (neural stem cells, dopaminergic neurons and mixed neurons, and astrocytes, that are ideal for comparative neuroscience studies, drug and toxicity screening. Our catalog of iPSC products also include differentiation and maintenance media and kits, to derive your own terminally differentiated cell lines from our iPSCs.
Advantages in choosing ASC's control iPSC lines:
- Fully characterized stem cell lines: expression of pluripotency markers, tertoma formation into tridermal layers and normal karyotype
- Suitable for genetic modification using CRISPR-Cas9, and as isogenic control for engineered cell lines
- Easy differentiation protocols to generate isogenic panels of neuronal lineage cells (dopaminergic and motor neurons, astrocytes and oligodendrocytes)
- iPSCs from multiple donors and tissue resources provide a broad genetic background for basic research, drug and toxicity screening application
Control-iPSC cell lines include:
- ASE-9203: iPSC-derived from normal human fibroblasts (Male)
- ASE-9109: iPSCs-derived from normal human cord blood (Male)
- ASE-9110: iPSCs-derived from normal human cord blood (Female)
Figure 1. Characterization data for Male cord blood cells derived iPSC line ASE-9109. Immunocytochemical staining for pluripotency markers NANOG, OCT4, TRA-1-60, and TRA-1-81.
Figure 2. Karyotype analysis for control iPSC line ASE-9109. Normal karyotype results for ASE-9109 control iPSC line derived from male cord blood CD34+ cells.
Figure 3. Characterization of control iPSC line ASE-9110 derived from female CD34+ cord blood cells. Immunocytochemical staining for pluripotency markers SOX2, NANOG, OCT4, TRA-1-60, and TRA-1-81.
We also have a well-characterized panel of patient-derived iPSC lines for Parkinson's disease, ALS and Diabetes research.
Custom iPSC Generation and Differentiation Services are also available. Please inquire
Differentiation of ASE-9109 control iPSC line into neural lineage cells
Figure 1. Neural lineage differentiation of control iPSC line ASE-9109 derived from male cord blood cells. Neural stem cells: Nestin/Sox1/DAPI; Mixed neurons: Tuj1 or GABA/ MAP2/ DAPI; Astrocytes: GFAP/DAPI.
Dopaminergic neurons differentiated from control iPSC line ASE-9109
Figure 2. Dopaminergic neurons differentiated from male cord blood control iPSC line ASE-9109. Immunocytochemical analysis of neuronal marker, Tuj1 (neuronal type β III tubulin; green); dopamine neuron marker, TH (tyrosine hydroxylase; red); and nuclear marker, DAPI (blue).
Frequently Asked Questions
1. What reprogramming method was used to generate the control iPSC lines ASE-9109 and ASE-9110?
These iPSC lines were derived from CD34-positive (CD34+) human cord blood cells using "integration-free" episomal reprogramming methods in feeder-free conditions.
Shaltouki, A., Sivapatham, R., Pei, Y., Gerencser, A. A., Momčilović, O., Rao, M. S., & Zeng, X. (2015). Mitochondrial alterations by PARKIN in dopaminergic neurons using PARK2 patient-specific and PARK2 knockout isogenic iPSC lines. Stem cell reports, 4(5), 847-859.
Efthymiou, A. G., Steiner, J., Pavan, W. J., Wincovitch, S., Larson, D. M., Porter, F. D., ... & Malik, N. (2015). Rescue of an in vitro neuron phenotype identified in Niemann-Pick disease, type C1 induced pluripotent stem cell-derived neurons by modulating the WNT pathway and calcium signaling. Stem cells translational medicine, 4(3), 230-238.
Efthymiou, A., Shaltouki, A., Steiner, J. P., Jha, B., Heman-Ackah, S. M., Swistowski, A., ... & Malik, N. (2014). Functional screening assays with neurons generated from pluripotent stem cell–derived neural stem cells. Journal of biomolecular screening, 19(1), 32-43.
Shaltouki, A., Peng, J., Liu, Q., Rao, M. S., & Zeng, X. (2013). Efficient generation of astrocytes from human pluripotent stem cells in defined conditions. Stem cells, 31(5), 941-952.