ESC tested Fetal Bovine Serum (FBS)
Embryonic stem cells require very stringent protocols for growth without differentiation. ESC-Sure™ ESC-qualified Fetal Bovine Serum (FBS) is a high quality, prescreened serum that is especially suited for use in embryonic and induced pluripotent stem cell (ESC, iPSC) culture. It strengthens the growth of ESCs with normal stem cell morphology while maintaining them in their undifferentiated and pluripotent state.
Our ESC-Sure™ FBS can provide the assurance scientists need for ES-cell culturing while saving time, money and effort. A certificate of analysis will be provided with each lot of ESC-Sure™ FBS.
- Quality tested for > 5 passages of mESCs
- Minimal endotoxin levels and hemoglobin content
- Low batch to batch variation
- Serum from aseptically collected blood in USDA-approved abattoirs
Figure. ESC-Sure™ FBS is extensively tested for supporting undifferentiated growth of mESCs. The image shows healthy mESCs that were cultured in a MEF-feeder cell system along with Applied StemCell’s ES-grade FBS.
Ideally, removing the bottle of serum from frozen storage and allowing to thaw overnight at room temperature (or 12 hours) is the best way. Homogenize the serum once thawed without making the serum foam. If you are not able to plan accordingly, you can also thaw the bottle of serum in a shaking/heated water bath set to 37⁰C until completely thawed.
Heat Inactivation degrades complement proteins that could interfere with specific assays. For most cell culture settings and applications, heat inactivation is not recommended. Effects of Heat Inactivation include a reduction in growth factors, increase of deposits which are commonly mistaken for microbial contamination and reduce performance of the serum.
- Chory, E. J., Kirkland, J. G., Chang, C. Y., D'Andrea, V., Gourisankar, S., Dykhuizen, E. J., & Crabtree, G. J. (2019). Inhibition of a Selective SWI/SNF Function Synergizes with ATR Inhibitors in Cancer Cell Killing. bioRxiv, 660456.
- Lv, Y., Xiao, F. J., Wang, Y., Zou, X. H., Wang, H., Wang, H. Y., ... & Lu, Z. Z. (2019). Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5. BMC biotechnology, 19(1), 23.
- Paynter, J. M., Chen, J., Liu, X., & Nefzger, C. M. (2019). Propagation and maintenance of mouse embryonic stem cells. In Mouse Cell Culture, vol 1940 (pp. 33-45). Humana Press, New York, NY.
- Chory, E. J., Calarco, J. P., Hathaway, N. A., Bell, O., Neel, D. S., & Crabtree, G. R. (2019). Nucleosome turnover regulates histone methylation patterns over the genome. Molecular cell, 73(1), 61-72.
- Gatchalian, J., Malik, S., Ho, J., Lee, D. S., Kelso, T. W., Shokhirev, M. N., ... & Hargreaves, D. C. (2018). A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells. Nature communications, 9(1), 5139.
- Chory, E. J., Calarco, J. P., Hathaway, N. A., Bell, O., Neel, D. S., & Crabtree, G. R. (2018). Nucleosome Turnover Regulates Histone Methylation Patterns over the Genome. Molecular cell.
- Marian, C. A., Stoszko, M., Wang, L., Leighty, M. W., de Crignis, E., Maschinot, C. A., ... & Duvall, J. R. (2018). Small Molecule Targeting of Specific BAF (mSWI/SNF) Complexes for HIV Latency Reversal. Cell Chemical Biology
Hodges, H. C., Stanton, B. Z., Cermakova, K., Chang, C. Y., Miller, E. L., Kirkland, J. G., ... & Crabtree, G. R. (2017). Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers. Nature Structural & Molecular Biology, 1.
Braun, S. M. G., Kirkland, J. G., Chory, E. J., Husmann, D., Calarco, J. P., & Crabtree, G. R. (2017). Rapid and reversible epigenome editing by endogenous chromatin regulators. Nature Communications, 8, 560.http://doi.org/10.1038/s41467-017-00644-y.s
Dykhuizen, E. C., Carmody, L. C., & Tolliday, N. J. (2017). High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR. In Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases (pp. 81-95). Humana Press, New York, NY.
Stanton, B. Z., Hodges, C., Calarco, J. P., Braun, S. M. G., Ku, W. L., Kadoch, C., … Crabtree, G. R. (2017). SMARCA4 ATPase mutations disrupt direct eviction of PRC1 from chromatin. Nature Genetics, 49(2), 282–288. http://doi.org/10.1038/ng.3735
For more references, visit our reference page..