• Stem Cell Culture : MEF cells, 3D Media, FBS

ESC tested Fetal Bovine Serum (FBS)

Embryonic stem cells require very stringent protocols for growth without differentiation. ESC-Sure™ ESC-qualified Fetal Bovine Serum (FBS) is a high quality, prescreened serum that is especially suited for use in embryonic and induced pluripotent stem cell (ESC, iPSC) culture. It strengthens the growth of ESCs with normal stem cell morphology while maintaining them in their undifferentiated and pluripotent state.

Our ESC-Sure™ FBS can provide the assurance scientists need for ES-cell culturing while saving time, money and effort. A certificate of analysis will be provided with each lot of ESC-Sure™ FBS.

  • Quality tested for > 5 passages of mESCs
  • Minimal endotoxin levels and hemoglobin content
  • Low batch to batch variation
  • Serum from aseptically collected blood in USDA-approved abattoirs
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Application Notes


Figure. ESC-Sure™ FBS is extensively tested for supporting undifferentiated growth of mESCs. The image shows healthy mESCs that were cultured in a MEF-feeder cell system along with Applied StemCell’s ES-grade FBS.

How do I thaw the serum?
Is heat inactivation necessary?

Applied StemCell's cited/ published journal articles:


  • Hodges, H. C., Stanton, B. Z., Cermakova, K., Chang, C. Y., Miller, E. L., Kirkland, J. G., ... & Crabtree, G. R. (2017). Dominant-negative SMARCA4 mutants alter the accessibility landscape of tissue-unrestricted enhancers. Nature Structural & Molecular Biology, 1.
  • Braun, S. M. G., Kirkland, J. G., Chory, E. J., Husmann, D., Calarco, J. P., & Crabtree, G. R. (2017). Rapid and reversible epigenome editing by endogenous chromatin regulators. Nature Communications, 8, 560.http://doi.org/10.1038/s41467-017-00644-y.s.
  • Dykhuizen, E. C., et al. (2017). High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR. Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases, 81-95.
  • Stanton, B. Z., et al. (2016). Nature Genetics. doi:10.1038/ng.3735.
  • Beske, PH., et al. (2016). Toxicological Sciences. 149(2):503-15. doi: 10.1093/toxsci/kfv254.
  • Miljan, E. Chapter 8: The business of stem cell research tools. Stem Cells in Regenerative Medicine: Science, Regulation and Business Strategies. (2015); pg149.
  • Hubbard, K., et al. Vis. Exp. (96), e52361, doi:10.3791/52361 (2015).
  • Beske, PH., et al. (2016). Toxicological Sciences. 149(2):503-15. doi: 10.1093/toxsci/kfv254.
  • Stanford Medicine Transgenic Research center (http://med.stanford.edu/transgenic/esmeflif.html).
  • Hathaway, N. A., et al. (2012). Cell,149(7), 1447-1460.
Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!