SNL 76/7 (STO Cell Line)

The SNL 76/7 cell line is derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. SNL 76/7 cells are commonly used as feeder cells for mouse and human ESC/iPSC culture. 

MEF cells serve as feeder cells to support the growth of undifferentiated mouse or human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). MEF cells are isolated from mouse embryos and should be used at an early passage. Before use as feeder cells, MEF cells must be mitotically inactivated by γ-irradiation or mitomycin-C treatment. 

The SNL 76/7 cell line is derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. SNL 76/7 cells are commonly used as feeder cells for mouse and human ESC/iPSC culture. 

All MEF cells are manufactured in the US and have an average viability of 95% after thawing. We offer both untreated cells for further expansion and treated cells that can be directly used as a feeder layer.

Also be sure to see our CF-1 MEF Feeder Cells,  DR4 MEF Feeder Cells,  and Neo-resistant MEF Feeder Cells.

SNL

Products and Services
Catalog ID#Product Name SizePriceQTY
$95.00
$48.00
$226.00
$48.00
$226.00
0.00

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Supporting Material

Brochures/ Flyers:

Icon-PDF Stem Cell Brochure

Publications

SNL 76/7 (STO Cell Line)

  • Yang, J., Ryan, D. J., Lan, G., Zou, X., & Liu, P. (2019). In vitro establishment of expanded-potential stem cells from mouse pre-implantation embryos or embryonic stem cells. Nature protocols, 1.
  • Kime, C., Rand, T. A., Ivey, K. N., Srivastava, D., Yamanaka, S., & Tomoda, K. (2015). Practical integration‐free episomal methods for generating human induced pluripotent stem cells. Current protocols in human genetics, 87(1), 21-2.
  • Takahashi, K., Narita, M., Yokura, M., Ichisaka, T., & Yamanaka, S. (2009). Human induced pluripotent stem cells on autologous feeders. PloS one, 4(12), e8067.
  • Park, I. H., & Daley, G. Q. (2009). Human iPS cell derivation/reprogramming. Current protocols in stem cell biology8(1), 4A-1.
  • Okita, K., Ichisaka, T., & Yamanaka, S. (2007). Generation of germline-competent induced pluripotent stem cells. Nature, 448(7151), 313.
  • Takahashi, K., Okita, K., Nakagawa, M., & Yamanaka, S. (2007). Induction of pluripotent stem cells from fibroblast cultures. Nature protocols, 2(12), 3081.
  • McMahon, A. P., & Bradley, A. (1990). The Wnt-1 (int-1) proto-oncogene is required for development of a large region of the mouse brain. Cell, 62(6), 1073-1085.

 

FAQ

Frequently Asked Questions

1. Can the SNL 76/7 (STO Cell Line) be expanded? Is it immortalized?

We don't recommend to passage cells more than P15, based on our experience.

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!