rESC/iPSC Pluripotency RT-PCR Kit

SKU
ASK-6201
Model
ASK-6201
Size
20 reactions
$100.00

Size                                       20 reactions

Contents                             6 pairs of primers

Concentration                  10 mM

Description

Two main properties of stem cells are their capacity for self-renewal and maintain pluripotency.  The expression of stem cell specific biomarkers has been generally accepted as one method to characterize the pluripotency of a stem cell line.  The most commonly used biomarkers that are used to identify the pluripotency of a rat ES/iPS cell line include Nanog, Oct4, Sox2, Eras and Rex1 [1,2].  Nanog, Oct4 and Sox2 are transcription factors that are highly expressed in undifferentiated ESC and embryonic germ cells (EGC)[3-5]. The expression of Eras and ZNF42 (Rex1) is significantly different between pluripotent and differentiated cells[1].

Content

 

Symbol

Accession ID

Primers

Size of PCR product

Application

rNanog

NM_001100781

rNanog

356 bp

ES cell marker

rOct4

NM_001009178

rOct4

412 bp

ES cell marker

rSox2

NM_001109181

rSox2

414 bp

ES cell marker

rEras

NM_001109375

rEras

501 bp

ES cell marker

rZnf42

NW_003812191

rZnf42

367 bp

ES cell marker

b-actin

 

b-actin

427 bp

Positive control