Pluripotency Markers (Protein)
ESC/iPSC Characterization Kit (Antibody Pluripotency Marker Kit)
Applied StemCell’s Embryonic Stem Cell (ESC) / Induced Pluripotent Stem Cell (iPSC) Characterization Kit is designed for users to analyze the pluripotent status of ESCs and iPSCs by analyzing marker protein expression. The kits are ready-to-use and provided a fast, convenient and sensitive method for confirming pluripotency in ESCs and iPSCs.
Applied StemCell's Pluripotency Marker Kit includes:
- Easy-to-use primary and secondary antibodies with well-established pluripotency markers to confirm the pluripotency of human or mouse ESC, iPSC lines.
- Fixation, Permeabilization, Blocking, and Mounting Reagents
- DNA counterstain
- Alkaline Phosphatase activity assay
Markers used in the kits:
- Human: OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81
- Mouse: OCT4, SOX2, SSEA1
- High Sensitivity: Partially differentiated hiPSC clones can be detected.
- Convenient: The ready-to-user kits contain all buffers and solutions needed to perform immunocytochemical and enzyme activity analysis.
Applied StemCell also offers mRNA Pluripotency Marker Kits which include primer sets to analyze expression of these markers as well as a positive control primer set.
The Pluripotency Marker Kit contains commonly used marker antibodies in prediluted, optimized concentration for immunofluorescence staining.
Oct4 and Sox2 are transcription factors highly expressed in undifferentiated both human and mouse ESCs/iPSCs and embryonic germ cells (EGC). SSEA-1 is a globoseries carbohydrate antigen present on the surface of murine ESC, but it is not detected in human pluripotent cells. SSEA4, TRA-1-60, TRA-1-81 are surface proteins specific for human ESCs/iPSCs.
In addition the kits provide a rapid and sensitive alkaline phosphatase (AP) activity test. AP is a stem cell membrane marker and elevated expression of AP is associated with the pluripotent status of ESCs/iPSCs.
Applied StemCell's cited/ published journal articles:
- Anti-SOX2 antibody:
- Wang, T., et al. (2015) J. Vis. Exp. (95), e52298, doi:10.3791/52298 (2015)
- Ramalingam, S., et al. (2013) Stem Cells Dev. 22(4):595-610.
- Grabundzija I (2012) Nucleic Acids Research, 1-19.
- Yang J (2014) Graefes Arch Clin Exp Ophthalmol: DOI 10.1007/s00417-014-2575-9.
Human ES-iPSC Characterization kit:
- Marei, H. E., Althani, A., Lashen, S., Cenciarelli, C., & Hasan, A. (2017). Genetically unmatched human iPSC and ESC exhibit equivalent gene expression and neuronal differentiation potential. Scientific Reports, 7(1), 17504
- Choi, J. (2015) Nat Biotech 33: 1173-1181
- Fleming, WH. (2015) US Patent 2015/0342577
- Li, Y., et al. (2012) Molecular Medicine 18, 1312-1319
- Li ,Y., et al. (2014) Molecular Therapy: 22(9):1688-97
- Yagyu, S., et al. (2015) Molecular Therapy. doi:10.1038/mt.2015.100
- Jing L (2012) Global Spine J 2012: 02-S4.18 DOI: 10.1055/s-0032-1319887
- Deuse, T., et al. (2011). J Cell Sci. 124(Pt 17):3029-37.
- Deuse, T., et al. (2014). Nature. 509(7502):641-4.
- Wang, J., et al. (2014) Nature 516: 405-409
- Wang, T., et al. (2013) Plos ONE 8(11): e81720.
- Zamule SM (2011) Chemico-Biological Interactions 190: 62-72
- Sun N (2013) Biotechnol. Bioeng. 9999: 1–6
- Tachibana, M., et al. (2013) Cell 153.6: 1228-1238.