TARGATT™ Plasmids

Applied StemCell offers a number of plasmids that can be used for our TARGATT technology. Each plasmid has a unique combination of promoters and marker genes to meet your special needs and to ensure robust transgene expression.

TARGATT™ 2 (CAG + Poly A)
TARGATT™ 3 (no promoter +  MCS)
TARGATT™ 6.1 (CAG-L4SL-MCS-PolyA)
TARGATT™ 7 (PGK-MCS-PolyA)
TARGATT™ 8 (PCA-MCS-PolyA)
TARGATT™ 9.1 (PCA-L4SL-MCS-PolyA)

 

Scheme for Gene Knock-in using a TARGATT™ Plasmid

Goal: To insert a GFP reporter gene into HCT116 cell line using the TARGATT 6.1 plasmid

One million HCT116 cells were transfected with 5 ug of TARGATT™ 6.1-GFP plasmid (CAG-L4SL-GFP), and with or without 2.5 ug Cre-expression plasmid using Neon® electroporation transfection system. In the presence of Cre recombinase expression, the LoxP-4stop-LoxP is deleted and GFP reporter is expressed. In absence of Cre-expression, the GFP expression is silenced by the 4 stop codons in LoxP-4stop-LoxP cassette. 

ProofofConcept-Targatt-plasmid-6.1

References

Description of the technology

Commentary, comparison with other transgenic methods

Tet inducible mice generated by TARGATT™

Advantage of Hipp11 (H11) locus

Applications for mice generated by TARGATT™

Ordering

Material Transfer Agreement (MTA) is required. Please contact us for more details.

Catalog ID#Product Name SizePriceQTY
$550.00
$550.00
$550.00
$550.00
$550.00
$550.00

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