TARGATT™ Transgenic Kit, 2 Rounds of Microinjections
Catalog # :
2 rounds of microinjections
The TARGATT™ Transgenic Kit is designed to create site-specific knock-in transgenic mice at a defined chromosomal locus in a more efficient and significantly faster way over traditional methods. Generating transgenic mice by conventional methods (e.g. pronuclear microinjection or lentiviral injection) has following limitations, first of which is random insertion of the transgene. Random insertion of a transgene results in position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Secondly, transgenes can be inserted as multiple copies, resulting in instability at the insertion locus.
The TARGATT™ Transgenic Kits is an essential component for the generation of TARGATT™ site-specific, knock-in transgenic mice. The kits are designed using the same highly optimized protocols that are used in our popular and successful Custom TARGATT™ Mouse Model Generation Service. The kits contain PhiC31 integrase mRNA, control mRNA and embryo-qualified miTE buffer, and are intended for the microinjection of a TARGATT™ donor plasmid (purchased separately) into the pronuclei of TARGATT™ “attP” mouse embryos.
The TARGATT™ Transgenic Kits offers an affordable, efficient and faster alternative for experienced scientists and transgenic core facilities to generate numerous site-specific knock-in mouse models in their own vivarium.
AST-1003 (Size: 5 reactions)
AST-1004 (Size: 2 reactions)
The kit contains:
- TARGATT™ Integrase mRNA* (20 μL/ vial): 2 vials (AST-1004) or 5 vials (AST-1003)
- Control mRNA (20 μL)
- Embryo-qualified miTE Buffer (1 mL)
- Detailed protocols on how to use the kit
* Transgene-integration tested. Each vial is good for one round of microinjection
Additional TARGATT™ components and kits needed but not provided with the kit:
- TARGATT™ plasmid to clone the donor DNA.
- TARGATT™ Mice for microinjection (purchase from ASC; shipped from Charles River)
- TARGATT™ Mouse Genotyping Kit