TARGATT™ Products for Site-Specific Knock-in Mouse Generation
Our proprietary site-specific DNA integration system, TARGATT™ lets you create site-specific transgenic mice in a more efficient and faster way compared to traditional methods. Generating a transgenic mouse (e.g. knock-in mouse) by conventional methods like pronuclear microinjection or lentiviral injection has several limitations, one of them being random insertion of the transgene. Random insertion of a transgene results in position effect where either the transgene is prone to silencing or endogenous gene expression is disrupted. Furthermore, random transgene insertion in the transgenic mouse usually happens in multiple copies, resulting in repeat induced silencing and genomic instability. The TARGATT™ technology uses PhiC31 integrase to insert any gene of interest into a specific docking-site that was pre-engineered into an intergenic and transcriptionally active genomic locus. Applied StemCell can create site-specific knock-in mice for you in as little as 3 months. Using our novel TARGATT™ system, a gene of interest can be inserted at a well-characterized, transcriptionally-active locus in the mouse genome with guaranteed transgene expression. Our scientists at Applied StemCell can create a TARGATT™ mouse for you, or you can purchase the animals and reagents to make your own.
Want more information on how to use TARGATT™ products for site-specific knock-in mouse generation?
View this webinar. It will describe the steps and how the technology works.
Fast! Reliable! Advantages of TARGATT™ Technology
- High integration efficiency mediated by PhiC31 integrase reduces time and cost.
- Site-specific integration at a pre-selected genomic locus eliminates position effect and ensures high expression levels of the transgene.
- Integration at intergenic region ensures that no internal genes are interrupted.
- Single copy gene integration eliminates repeat-induced gene silencing and genomic instability.
- Site-specific integration allows a precise comparison of the effects of the transgenes among different lines.
Ask us for a list of core facilities currently using the TARGATT™ system, including UCSF, NIH, MaxPlank, Harvard, Colombia and many more!
This transgenic mouse line contains an attP site at the H11 loci for easy site-specific gene knock-in and is available for purchase from Charles River. Make your own TARGATT™ knock-in mouse for gene overexpression, reporter gene insertion, knock-down by siRNA, humanized models and other applications. TARGATT™ attP Mice are available in the FVB and C57BL6 backgrounds.
Our TARGATT™ Embryos can be used to establish your own TARGATT™ Transgenic Mouse colony via embryo transfer. We offer high quality H11 (Hipp11) and ROSA26 TARGATT™ Embryos (Frozen) which contain an attP site in either H11 or ROSA26 locus. Both loci are available in two backgrounds, FVB and C57BL6. After setting up your TARGATT™ Transgenic Mouse colony, you can generate site-specific knock-in mice using our TARGATT™ Transgenic Kit.
TARGATT™ Transgenic Kits are essential for the generation of TARGATT™ knock-in mice. The kits contain PhiC31 integrase mRNA, control mRNA and buffer and are designed for the microinjection of a TARGATT™ plasmid into the pronuclei of TARGATT™ Transgenic Mouse embryos.
We offer a wide range of plasmids for our TARGATT™ Transgenic Knock-in Mouse applications. Our TARGATT™ plasmids are engineered to contain various promoter and marker gene combination to best meet your needs when generating your transgenic mouse.
The TARGATT™ Mouse Genotyping Kit allows for convenient genotyping of TARGATT™ Transgenic Mice and knock-in mice generated from TARGATT™ Embryos via tail biopsy. Use this kit to confirm the presence of an attP knock-in site (H11 or ROSA26) and/or the integration of a transgene into the TARGATT™ Site.