PD-1 & CTLA4

SKU :
ASHU-00079
Catalog # :
ASHU-00079
Availability :
Mouse

PD-1 & CTLA4

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Strain Information
  • Catalog Number ASHU-00079
  • Model Name PD-1 & CTLA4
  • Strain Name C57BL/6 - Pdcd1em1(hPDCD1)Ctla4em1(hCTLA4)/Asc
  • Mutation Type Humanized
  • GENE Synonyms PD-1, Pdc1, Ly101, Cd152, Ly-56, Ctla-4
  • Human Orthologs PDCD1, CTLA4
  • Emsamble ID ENSMUSG00000026285, ENSMUSG00000026011
  • MGI ID 104879, 88556
  • NCBI ID 18566,12477
  • Background C57BL/6
  • Coat Color Black
  • Mutation Type Humanized
Description

Generation Strategy

Strategy for Generating Humanized PD-1 (ASHU-00015) Mouse Model

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Strategy for Generating Humanized CTLA4 (ASHU-00014) Mouse Model

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The Expression of hPD-1 in Stimulated T Cells from Double Humanized PD-1 & CTLA4 Mice 

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Figure 1. The expression of human CTLA-4 in double humanized PD-1 & CTLA-4 mice was confirmed by FACS.

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Figure 2. In vivo validation of double humanized PD-1 & CTLA-4 mice. The Double humanized mice were inoculated with MC38 cells, and randomly assigned to different groups (n=8) when the tumor grew to a volume of 100 mm3. A combinatorial treatment of anti-CTLA-4 and anti-PD-1 demonstrated a noticeable improvement in efficacy as compared to the same dose of single agent (top figure) without affecting the animal body weight (bottom).

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Figure 3. As shown in the previous figure, animals of group 1-4 received different doses of either single agent or combinatorial treatment. This graph showed the progression of the tumor implanted in the cured, G4 mice, implicating a systematic anti-tumor effect induced by the combinatorial antibody treatment. 

Research Application
Immunotherapy, cancer research, drug screening
Availability
Mouse
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