4-1BB - Immune Checkpoint Mouse Models - Mouse Repository - Animal Models - Research

SKU :
ASHU-00099
Catalog # :
ASHU-00099
Availability :
Mouse
Knock-in Strategy Figure 1. Gene knock-in strategy to engineer a mouse model expressing a chimeric human-murine 4-1BB (also called CD137, TNFRSF9) gene. The endogenous mouse Tnfrsf9 gene was replaced with a human TNFRSF9 extracellular and mouse Tnfrsf9 transmembrane-intracellular (cytoplasmic) region downstream of the ATG start codon. 4-1BB (CD137) Expression in Humanized Mouse Model Figure 2. Flow cytometric analysis of pan T cells isolated from male heterozygous humanized 4-1BB mice (hC .....
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Strain Information
  • Catalog Number ASHU-00099
  • Model Name 4-1BB
  • Strain Name C57BL/6J - Tnfrsf9em1(hTNFRSF9) Asc
  • Mutation Type Humanized
  • GENE Synonyms ILA; Ly63; 4-1BB; Cd137; CDw137; AA408498; AI325004; A930040I11Rik
  • Human Orthologs TNFRSF9
  • Emsamble ID ENSMUSG00000028965
  • MGI ID 1101059
  • NCBI ID 21942
  • Backgound C57BL/6J
  • Coat Color Black
  • Mutation Type Humanized
Description

Knock-in Strategy

Landingpage-ASHU-00099-4-1BB-HU-ICPMouse-1.01

Figure 1. Gene knock-in strategy to engineer a mouse model expressing a chimeric human-murine 4-1BB (also called CD137, TNFRSF9) gene. The endogenous mouse Tnfrsf9 gene was replaced with a human TNFRSF9 extracellular and mouse Tnfrsf9 transmembrane-intracellular (cytoplasmic) region downstream of the ATG start codon.

4-1BB (CD137) Expression in Humanized Mouse Model

Landingpage-ASHU-00099-4-1BB-HU-ICPMouse-2

Figure 2. Flow cytometric analysis of pan T cells isolated from male heterozygous humanized 4-1BB mice (hCD137+/-). Male spleen pan T cells were isolated and stimulated with 1µg/mL of anti-CD3 and 1µg/mL of anti-CD28 for 48 hours. The cells were stained with anti-hCD137-APC, mCD137-PE, mCD3-V450 and mCD8-FITC for flow cytometry analysis. Results showed that human 4-1BB (hCD137) was expressed in both activated CD4+ and CD8+ lymphocytes.

Research Application
Immunotherapy
Availability
Mouse
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