Validation of Humanized PD-1 Expression by RT-PCR and Western Blot
Humanized PD-1 Expression in Spleen T Cells after Stimulation
Expression of h4-1BB and m4-1BB on CD3/CD28-activated T Lymphocytes from h4-1BB Heterozygous Mice
Male spleen pan-T cells isolated from the humanized 4-1BB heterozygous mouse were stimulated with 1g/mL of a-mCD3 and 1g/ml of a-mCD28 for 48 hours. The cells were stained with anti-hCD137-APC, mCD137-PE, mCD3-V450 and mCD8-FITC for flow cytometry analysis.
In Vivo Validation hPD-1 Mouse in a Syngenic MC38 Colon Cancer Model Using Anti-PD-1 Antibodies