4-1BB (2)

SKU :
ASHU-190077
Catalog # :
ASHU-190077
Availability :
Mouse

4-1BB (2)

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Strain Information
  • Catalog Number ASHU-190077
  • Model Name 4-1BB (2)
  • Strain Name C57BL/6-Tnfrsf9tm2(TNFRSF9)Asc
  • Mutation Type Humanized
  • GENE Synonyms ILA, Ly63, 4-1BB, Cd137, CDw137, AA408498, AI325004, A930040I11Rik
  • Human Orthologs TNFRSF9
  • Emsamble ID ENSMUSG00000028965
  • MGI ID 1101059
  • NCBI ID 21942
  • Background C57BL/6
  • Coat Color Black
  • Mutation Type Humanized
Description

Humanized Strategy

Landingpage-ASHU-190077-4-1BB-HU-ICPMouse-1

Validation Data

Landingpage-ASHU-190077-4-1BB-HU-ICPMouse-2.01

Figure 1. Expression of humanized CD137 (4-1BB) in the activated spleen lymphocytes of heterozygous humanized 4-1BB mice is detected by FACS. 

The spleen lymphocytes of heterozygous humanized 4-1BB mice were activated by anti-CD3 and anti-CD28 for 48 hours, and then collected for staining. The expression of humanized 4-1BB was detected by FACS. The results showed that the active expression of humanized 4-1BB can be detected in both activated CD4+ and CD8+ T lymphocytes collected from heterozygous humanized 4-1BB mice.

 (Data in partnership with collaborators)

landingpage-ashu-190077-4-1bb (2)-hu-icpmouse-2.02

Figure 2.  Analysis of human 4-1BB (CD137) expression in the spleen by FACS. The homozygous KI mouse expresses human 4-1BB on the CD8+ T cells.

Landingpage-ASHU-190077-4-1BB-HU-ICPMouse.3 

Figure 3.In vivo validation of humanized 4-1BB mice. Humanized 4-1BB mice were inoculated with MC38 colon cancer cells, and were randomly assigned into different groups (n=6) when the tumors grew to a volume of 100 mm3. Human-specific, 4-1BB antibody ( Urelumab or Utomilumab) was given every three days (Q3D) for two weeks.

(Data in partnership with collaborators)

Research Application
Immunotherapy, cancer research, drug screening
Availability
Mouse
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