Prkdc and Il2rg genes were both knocked-out in NOD mice.
- Lacking mature T, B and NK cells
- Low immune rejection against human cells and tissues
- Good tumorigenicity so that a small number of cells can form tumors.
- Significant improvement in the survival of transplanted human cells and tissues
- Suitable for the transplantation of human hematopoietic stem cells and the preparation of humanized mouse models
- Suitable as the carrier mice for the transplantation of heterologous cells and tissues
FACS detection of T, B and NK cells collected from M-NSG mice
Figure 1. Complete deletion of T, B and NK cells in the spleen of M-NSG mice.
(A) The splenocytes of BALB/c, NOD-scid and M-NSG mice were collected to analyze their compositions of T, B and NK cells by FACS. (B) Statistical analysis of sorted cells.
Figure 2. Complete deletion of T, B and NK cells in the blood of M-NSG mice.
(A) The peripheral blood samples of BALB/c, NOD-scid and M-NSG mice were collected to analyze their compositions of T, B and NK cells by FACS. (B) Statistical analysis of sorted cells.
Figure 3. Compositions of monocytes, macrophages, DCs in the spleen of M-NSG mice.
(A) The splenocytes of BALB/c, NOD-scid and M-NSG mice were collected to analyze their compositions of monocytes, macrophages, DCs. (B) Statistical analysis of sorted cells.
Figure 4. Compositions of monocytes, macrophages, DCs in the blood of M-NSG mice.
(A) The peripheral blood samples of BALB/c, NOD-scid and M-NSG mice were collected to analyze their compositions of monocytes, macrophages, DCs. (B) Statistical analysis of sorted cells.
Histological sections of spleen from M-NSG mice
Figure 5. Spleen from M-NSG mouse shows complete loss of white pulps (10×). Compared with C57BL/6 mice, the spleen of M-NSG mice lacked white pulp containing a large number of lymphocytes and the thymus are atrophied.
Figure 6. Histological sections of various tissues from M-NSG mice. Furthermore, no significant abnormalities were observed in other tissues, including brain, retina, spinal cord, heart, liver, lung, kidney, small intestine, large intestine, stomach, salivary glands, pancreas, ovary, uterus, testis, epididymis, skeletal muscle, skin, and brown fat.