C57BL/6J-Cyp2e1em1(CreERT2)Asc - Other Gene Edited Mouse Models (KO, CKO) - Mouse Repository - Animal Models - Research

SKU :
ASKI-00047
Catalog # :
ASKI-00047
Availability :
Mouse
The Cyp2e1 is a member of the CYP2 subfamily of cytochrome P450 enzymes which is responsible for many reactions involved in drug metabolism, synthesis of cholesterol, steroids and other lipids in humans. The Cyp2e1 is mainly expressed in hepatocytes and plays an important role in alcohol and toxin metabolism. The Cyp2e1-CreERT2 mouse model was generated using CRISPR/Cas9 and homology directed repair (HDR) where the CreERT2 recombinase sequence was inserted downstream of the Cyp2e1 5’ UTR .....
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Strain Information
  • Catalog Number ASKI-00047
  • Model Name Cyp2e1-(CreERT2)
  • Strain Name ASKI-00047
  • Mutation Type Knock-in
  • GENE Synonyms Cyp2e
  • Human Orthologs CYP2E1
  • Emsamble ID ENSMUSG00000025479
  • MGI ID 88607
  • NCBI ID 13106
  • Backgound C57BL/6J
  • Coat Color Black
  • Mutation Type Knock-in
Description

The Cyp2e1 is a member of the CYP2 subfamily of cytochrome P450 enzymes which is responsible for many reactions involved in drug metabolism, synthesis of cholesterol, steroids and other lipids in humans. The Cyp2e1 is mainly expressed in hepatocytes and plays an important role in alcohol and toxin metabolism.

The Cyp2e1-CreERT2 mouse model was generated using CRISPR/Cas9 and homology directed repair (HDR) where the CreERT2 recombinase sequence was inserted downstream of the Cyp2e1 5’ UTR in the mouse endogenous gene locus and its expression is controlled by the Cyp2e1 promoter. This mouse model enables the generation of liver-specific tamoxifen-inducible conditional knockout or expression mouse model.

 

Validation of Humanized Mouse Model

Knock-in Strategy

ASKI-00047 

Figure 1. Gene knock-in strategy to engineer Cyp2e1-CreERT2 mouse line. Briefly, a mixture of Cas9 mRNA, gRNA targeting the 5’ UTR-exon 1 region of mouse Cyp2e1 locus, and the donor vector containing the CreERT2 cassette was microinjected into fertilized eggs from the wild type C57BL/6J mice. The founder mice (F0) were identified by PCR and sequencing. The F0 mice were then crossed with wild type C57BL/6J mice to generate germline transmitted Cyp2e1-CreERT2 F1 mice.

 

PCR Genotyping of Heterozygous F1 Knock-in Mic

ASKI-00047

Figure 2. Genotyping of F1 germline transmitted heterozygous Cyp2e1-CreERT2 mice. PCR using primer sets for the 5’ arm and 3’ arm were used to confirm insertion of the Cre-ERT2 downstream of the Cyp2e1 promoter in the mouse genome. LEFT: Agarose gel electrophoresis of the 5’ arm PCR was positive for the transgene integration with an expected 5.3 kb fragment. The is no fragment for the wild type allele. RIGHT: Agarose gel electrophoresis of 3’ arm PCR showed a 3.5 kb fragment indicative of transgene integration and a 7.7 kb wild type fragment in four heterozygous F1 mice. MIDDLE: M; 1 kb DNA ladder.

Research Application
Immunotherapy
Availability
Mouse
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