Tgtn (CAG-human ACE2-IRES-Luciferase) - Mouse Repository - Animal Models - Research

SKU :
ASTG-200002
Catalog # :
ASTG-200002
Availability :
Mouse
Strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) Transgenic Mouse Model Figure 1: Construction strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model Plasmid Map Figure 2: Plasmid map of the TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model targeting vector Vector Evaluated by Restriction Endonucleases Figure 3: Agarose gel electrophoresis of the vector digested by EcoRI. Lane 1 represents three fragments of length 7805bp, .....
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Strain Information
  • Catalog Number ASTG-200002
  • Strain Name C57BL/6J-Tgtn(CAG-human ACE2-IRES-Luciferase-WPRE-polyA)Asc
  • Mutation Type Transgene
  • Human Orthologs ACE2
  • Mutation Type Transgene
Description

Strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) Transgenic Mouse Model

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Figure 1: Construction strategy of TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model

Plasmid Map

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Figure 2: Plasmid map of the TgTn (CAG-human ACE2-IRES-Luciferase-WPRE-polyA) transgenic mouse model targeting vector

Vector Evaluated by Restriction Endonucleases

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Figure 3: Agarose gel electrophoresis of the vector digested by EcoRI. Lane 1 represents three fragments of length 7805bp, 2703 bp and 415bp in the EcoRI digest. M: 1 kb DNA ladder (from Fermentas).

PCR Genotyping Result of Positive F0 Generation

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Figure 4: PCR genotyping result of positive F0 generation. A. Genotyping Strategy of F0 mice: TG=819 bp, WT=0 bp. B. Agarose gel electrophoresis of PCR products: Ten positive F0 generation mice were obtained (Nos.: 17, 18, 21, 22, 23, 30, 35, 38, 40, 47). Abbreviations: Nos.: positive F0 mouse ID numbers; TG: Transgenic; WT: wide type; M: 1kb DNA ladder from Fermentas)

PCR Genotyping Method for Identifying F0 Generation

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Luciferase Expression in Transgenic Mouse Model

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Figure 5: Detection of luciferase expression in C57BL/6J-TgTn(CAG-human ACE2-IRES-Luciferase-WPRE-polyA) mice. The images shown are representative images are from the abdomen (A) and back (B). Mice with ID No. 17, 21, 22, 30, 35, 38, 40, 47 showed strong fluorescence. The color scale in intensity/sec is shown on the right.

Research Application
Corona virus related research
Availability
Mouse
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