TARGATT™ Genome Engineering for Animal Bioproduction
Animal bioproduction uses transgenic animal mammary gland as a bioreactor for the production of recombinant proteins. Animal bioproduction, compared to CHO bioproduction, has the advantages of lower upfront and maintenance costs, is easy to contain, control and transport, involves faster development processes and no scale-up issues, and has a unique low cost bulk holding stage (frozen milk). Existing methods used to create transgenic animals for the bioproduction of important recombinant proteins, has many limitations. For example, target genes are randomly inserted, there is no control of copy number and the yield of recombinant protein is low, usually between 1-2g/L. In addition, each new protein is a new project with different and unpredictable outcomes.
Applied StemCell has pioneered a new method to generate transgenic animals using our proprietary TARGATT™ technology. This technology allows us to reproducibly, and consistently, control the knock-in locus, copy number and expression of the target protein. TARGATT™ utilizes the PhiC31integrase and attP docking sites to direct the chosen target protein, with high efficiency, to a pre-selected and well characterized locus. Our method ensures high expression and eliminates position effects such as gene silencing, genomic instability and interruption of endogenous genes.