CRISPR Knockout Cell Line Service

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ASC provides comprehensive custom service to generate CRISPR knockout cell lines using its successful CRISPR/Cas9 cell line generation platform. With >1300 unique cell line models generated to-date and in >200 distinct mammalian cell lines, we can generate a knockout cell line model to your specifications, in as little as 2 months.

  • Optimized gene knockout protocols for:
    • Frameshift mutation
    • Fragment excision
    • Stop cassette insertion
    • Double gene knockout
  • Homozygous/ heterozygous knockout clones
  • Variety of cell lines: cancer, hard-to-transfect, most mammalian species
  • Workflow includes:
    • gRNA design, construction, and validation
    • Donor DNA construction (if needed)
    • Cell line validation, transfection and optimization
    • Screening for single cell clones and clone confirmation
    • Cell expansion and cryopreservation


  • Two homozygous (or heterozygous) clones with correct gene mutation for the modified line; two vials of each clone; 1 x 10^6 cells/vial.
  • Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-4 months


  • Disease modeling for immuno-oncology, pharmacogenomic studies
  • Drug discovery and drug efficacy and toxicity screening; drug combination studies
  • Antibody validation
  • Deriving diagnostic reference standards and materials
  • Recombinant protein production in CHO cells
  • Generation of TARGATT™ master cell lines by inserting an attP "docking site" for site-specific gene knock-in