CRISPR Custom Point Mutation Cell Line Generation Service

SKU :
ASC-6012-3
Catalog # :
ASC-6012-3
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Overview

Generate physiologically relevant in vitro model with mutations seen in clinical specimens in your preferred cell lines. ASC can knock-in or correct point mutations in your gene of interest in control or disease cell lines. Very ideal for immune-oncology research and drug discovery, our CRISPR/Cas9 cell line model generation protocols can generate precise point mutations in the targeted locus.

  • With or without silent mutation; footprint-free genome editing capable
  • Homozygous/ heterozygous variant clones
  • Variety of cell lines: cancer, hard-to-transfect, most mammalian species

Workflow includes:

  • gRNA design, construction, and validation
  • Donor DNA construction
  • Cell line validation, transfection and optimization
  • Screening for single cell clones and clone confirmation
  • Cell expansion and cryopreservation

Deliverables:

  • Two homozygous clones with correct gene mutation for the modified line; two vials of each clone; 1 x 10^6 cells/vial.
  • Milestone update/report; final report with detailed description of each procedure, including targeting vector design, construction and validation, transfection condition, genotyping strategy, and results.

Timeline: 3-4 months

Applications:

  • Disease modeling for immuno-oncology, pharmacogenomic studies
  • Drug discovery and drug efficacy and toxicity screening; drug combination studies
  • Deriving diagnostic reference standards and materials