Patient Fibroblasts Immortalization Service
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Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types and the mechanism of SV40 T antigen in cell immortalization is relatively well understood. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells. Here the human fibroblasts were immortalized by introducing SV40 T antigen.
- Lentivirus vector(s) expressing SV40 large and small T antigens
- Transduction, colony selection (up to 10 passages)
- Characterization (transgene expression by RT-PCR)
- Culture, cryopreservation
Some primary cells may encounter what’s known as a “crisis stage” after over ~25-30 passages. Therefore, truly immortalized cell lines must be cultured long-term to pass the “crisis stage.” Our guarantee includes the positive transduction and expression of T antigen in target cells. If required, ASC will add the long-term culture to test beyond the “crisis stage” option for additional charge, which will be estimated based on the growth properties of the transduced cells; please contact us for an additional quote.