• Cell-Based Drug Toxicity and Efficacy Testing

Cell-Based Drug Toxicity and Efficacy Testing

Make Informed Go-No-Go Decisions Early in Your Drug Development! Process! Applied StemCell (ASC), an ISO-certified service provider offers a flexible assay platform with a wide-range of functional endpoints for early-stage in vitro screening of preclinical drug candidates. We use a comprehensive cell-based test battery from which you can choose assays for efficacy, safety or target discovery that suit your therapeutic pipeline. We will provide the scientific expertise, accurate and efficient screening to help you take informed decisions about your small/ large molecules early in your drug development process to avoid costly drug attrition. Our custom screening service is ideal for CNS, cardiovascular, metabolic, cancer, and immunotherapy drug candidates.

Technical Details

1. In Vitro Disease Modeling

Genome Editing/ Correction
 (Cancer cells, iPSCs, ESCs, primary cells)
iPSC Reprogramming & Differentiation 3D Cell/ iPSC Culture
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2. Drug Screening

Phenotypic Screening Functional Validation Cell Viability/ Toxicity Mitochondrial/ Hepatic/Cardio Toxicity Customized Assay Development

1. In Vitro Disease Modeling:

Screen your compounds in a wide-variety of cell lines! Applied StemCell is a market leader in gene editing technologies. We can engineer > 100 distinct cell lines with a wide variety of mutations, using a combination of different technologies (CRISPR/Cas9, TARGATT™ and more) and techniques (transfection/transduction) to generate many different cell line models that are perfectly suited to screen candidate compounds in your therapeutic pipeline.

 We also provide comprehensive iPSC services and products for iPSC-based neurotoxicity & efficacy screening:

2. Drug Screening:

Flexible/ customizable project options to suit your screening needs! Below is a sample list of some of the assays we offer.

(Don’t see an assay you want? CONTACT US with your project requirements.)

Screening

Types of Assays

Estimated Timeline

Cytotoxicity & Cell Viability Assays

MTT/ MTS cell proliferation assay

LDH, Necrosis and Apoptosis assays

Luciferase (bioluminescence) expression

cAMP level measurement

4-6 weeks

Mitochondrial Toxicity Testing

Enzyme activity

Volume fraction detection

2-4 weeks

Functional Assays

Calcium influx/ imaging

Electrophysiology: Multielectrode array (MEA) analysis and Patch clamp recording

8-12 weeks

Quantitative Gene Expression

qPCR

RNA-seq using NGS (next generation sequencing)

2-8 weeks

Morphology

Neurite growth assay

Biomarker screening

2-4 weeks

Custom Assays

iPSC generation; characterization; gene editing; differentiation

Custom assay development

Based on project requirements

Custom Service Project Workflow:

Introduction to
Project Manager

- Primary point of contact during the course of the project
- Coordinates across scientific groups, and provides updates and official reports on project progress.

Project Kick-off
Meeting

- Preliminary meeting with PM and scientists
- Ensure that objectives and expectations are aligned

Bi-weekly to Monthly
Conference Calls

- Regular meetings with PM and scientists
- Opportunity to communicate new data, troubleshoot challenges, and if necessary, adjust project specifications

Milestone and
Final Reports

- Finalize data transfer for intermediate milestones and overall project
- Work with client to transfer final deliverables

CONTACT US

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!