CRISPR Knockout iPSC Service

SKU :
ASC-6013-1
Catalog # :
ASC-6013-1
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Overview

Applied StemCell is a leader in both CRISPR genome editing and iPSC technologies. We offer a combination CRISPR iPSC/ ES Cell Genome Editing Service with both these technologies to offer. With > 500 unique iPS cell line models generated to-date and a >98% success rate, leverage our unique expertise. We can generate gene knockout in your gene of interest.

Also available, downstream iPSC differentiation and characterization services.

Other features include:

  • Frameshift or target exon deletion; Large fragment deletion (>10 kb)
  • Homozygous (or heterozygous) knockout clones
  • Footprint-free, feeder-free transfection and iPSC culture protocols
  • Isogenic control lines for reliable experiments

Workflow includes:

  • Cell line evaluation
  • gRNA design, construction, and validation
  • Transfection and optimization
  • Screening for single cell clones and clone confirmation
  • Cell expansion and cryopreservation

Deliverables:

  • Two (2) clones that are homozygous (or heterozygous) for the targeted null allele, with two (2) vials of each clone at 1 x 10^6 cells/vial.
  • Project Milestones Reports, as well as a Final Report containing details of targeting design, experimental, and genotyping results.

Timeline: 3-4 months

Applications:

  • Physiologically relevant disease models for hard-to-model diseases (Ex. ALS, muscular dystrophy, Parkinson's disease, Alzheimer's)
  • Differentiate to study mutations in different tissue lineages with an isogenic panel of cell line models
  • Ideal for target drug discovery, drug and toxicity screening