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Teratoma Formation Analysis - Stem Cells - iPSC and ESCs - Cell Line Models - Research

SKU :
ASC-6008-1
Catalog # :
ASC-6008-1
Questions ? Contact Us
Description
Service to confirm pluripotency of human and mouse ESC and iPSC lines by Teratoma Formation Analysis after injection of cells into an animal.
Features:
- Turnaround time: 4 weeks (Mouse), 7 weeks (Human)
- Number of cells required / injection site: Mouse ESCs/iPSCs = 0.5 x 10^6, Human ESCs/iPSCs = 1 x 10^6
- Enhanced experimental protocol: Injection under both the kidney capsule and in testis results in higher efficiency of distinct teratoma formations with differentiated tissues derived from all three germ layers.
Service Includes:
- Comprehensive report with histological analysis and high resolution publication-grade images of endoderm, mesoderm and ectoderm formation.
- Tissue blocks and H&E stained tissue section slides
Notes:
- Frozen sample culture Fee $895 per line (No fee for fresh cells)
- Pathogen tests (please provide mycoplasma test result showing negative; otherwise additional testing fee will be required.)
- Kidney capsule tumor and testis tumor from each mouse will be embedded in one block. Separate blocks are available upon request with additional fee.
- Frozen tissue blocks are available with additional fee.
Technical Details:
- Experimental setup: A total of 4 mice (3 experimental and 1 control) will be used.
- Injection site: Each recipient mouse will be injected at two locations: in the kidney capsule and in the testis.
- Service includes: Cell injection, teratoma harvesting, tissue preparation (embedding and sectioning), H&E staining and histology analysis of teratoma tissue sections.
High Teratoma Formation Rate:
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Published reports of ASC's Teratoma Formation Analysis Reports can be found in the Certificate of Analysis "Induced Pluripotent Stem Cells (iPSCs)" by Coriell Institute for Medical Research.
References:
- Zhang WY, Teratoma formation: A tool for monitoring pluripotency in stem cell research. StemBook.
- Valamehr B (2012) SCIENTIFIC REPORTS 2: 213
- K. Chen et al., Stem Cell Research USA. 9, 237–248 (2012)
- Telugu BPVL (2011) JOURNAL OF BIOLOGICAL CHEMISTRY 286:28948-28953
- Deleidi M (2011) Stem Cells 29: 1052–1063
- Cooper O (2012) Sci Transl Med. 4(141):141ra90
- Zheng Z (2012) Biomaterials 33: 5821-5831
- Almeida S (2012) Cell Report 2: 789-798