• CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

We leveraged our expertise in CRISPR/Cas9 technology to develop affordable and convenient “do-it-yourself” kits for generating your own genetically modified human and mouse cell line models. The CRISPRCLEAR™ kits for cell line model generation are available in two formats: (1) Custom designed kits for knock-in, knockout or point mutation models for a specific genomic locus; (2) Ready-to-use kits for transgene knock-in into a safe harbor locus in any cell line, including iPSCs, blood-lineage cells and mouse cell lines. These kits are manufactured in our ISO:9001 quality management system certified facility in the USA using very optimized protocols and stringent quality control criteria.

CRISPR/Cas9 Genome Editing Categories

Cell Line Model Generation

Our CRISPRCLEAR™ Cell Line Model kits offer an affordable and efficient way to genetically engineer human or mouse cell lines in your own laboratory.

Cell Line Model Generation

Products and Services

9 Items

per page
Publications

CRISPR Cell Line Genome Editing

  • Selvan, N., George, S., Serajee, F. J., Shaw, M., Hobson, L., Kalscheuer, V. M., ... & Schwartz, C. E. (2018). O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling. Journal of Biological Chemistry, jbc-RA118.

  • Smalley, E. (2018). FDA warns public of dangers of DIY gene therapy.https://doi.org/10.1038/nbt0218-119

  • Chai, S., Wan, X., Ramirez-Navarro, A., Tesar, P. J., Kaufman, E. S., Ficker, E., ... & Deschênes, I. (2018). Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity. The Journal of clinical investigation128(3).

  • Boi, S., Ferrell, M. E., Zhao, M., Hasenkrug, K. J., & Evans, L. H. (2018). Mouse APOBEC3 expression in NIH 3T3 cells mediates hypermutation of AKV murine leukemia virus. Virology518, 377-384. https://doi.org/10.1016/j.virol.2018.03.014.

  • Molinski, S. V., et al. (2017). Orkambi® and amplifier cotherapy improves function from a rare CFTR mutation in geneedited cells and patient tissue. EMBO Molecular Medicine, e201607137.

  • Petrovic, P. B. (2017). Myosin Phosphatase Rho-interacting Protein Regulates DDR1-mediated Collagen Tractional Remodeling (Doctoral dissertation, University of Toronto (Canada).

  • Peng, L., Zhang, H., Hao, Y., Xu, F., Yang, J., Zhang, R., ... & Chen, C. (2016). Reprogramming macrophage orientation by microRNA 146b targeting transcription factor IRF5. EBioMedicine14, 83-96.

  • Hu, J. K., Crampton, J. C., Locci, M., & Crotty, S. (2016). CRISPR-mediated Slamf1Δ/Δ Slamf5Δ/Δ Slamf6Δ/Δ triple gene disruption reveals NKT cell defects but not T follicular helper cell defects. PloS one11(5), e0156074.

  • Smalley, E. (2016). CRISPR mouse model boom, rat model renaissance. Nature Biotechnology. 34, 893–894.

  • Baker, M. (2014). Gene editing at CRISPR speed. Nature biotechnology32(4), 309-313.

CRISPR Technology:

AAVS1 locus

  • Varshney, GK., et al. (2015). Genome research, 25(7), 1030-1042.

  • Tiyaboonchai, A., et al. (2014) Stem cell research, 12(3), 630-637

  • Sadelain, M., et al. (2012). Cancer, 12(1), 51-58.

H11 locus

Rosa26 locus

Technical Details

SCHEMATIC-CRISPR

We also offer products and kits using our proprietary TARGATT™ technology for efficient, site-specific gene insertion. The TARGATT™ products are an ideal toolkit for efficient knock-in of large transgenes which are usually not very efficient using CRISPR/Cas9.

Choose the right gene editing technology

Applied StemCell also uses a propriety site-specific integrase-based gene editing technology, TARGATT™ for large fragment (up to 20 kb) knock-in in safe harbor locus. The complementary TARGATT™ and CRISPR/Cas9 technologies can be used together to generate advanced cell line and animal models very efficiently and effectively.

Project Purpose

CRISPR/Cas9

TARGATT™

Knock-Out (KO)

Yes

 

Point Mutation

Yes

 

Conditional KO

Yes

 

Knock-In

(<200 Nucleotide ssODN Donor)

Yes

 

Knock-In Transgenes in

Safe Harbor Loci (>2kb)

Challenging

(but limitations on size)

Yes

 (up to 20kb)

Knock-In

 (Plasmid DNA)

Challenging

(but limitations on size)

Yes

 (2 steps: KI docking site; KI transgene) 

Have Questions?

An Applied StemCell technical expert is happy to help, contact us today!