CRISPR/Cas9 Genome Editing

 CRSIPR/Cas9 Genome Editing

 

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Applied StemCell is one of the earliest licensees of CRISPR technology (from the Broad Institute) and one of the long-time providers of custom CRISPR/ Cas9 gene editing services. Using our extensive CRISPR expertise in successfully completing > 500 animal and cell line model projects, we have developed a series of affordable and straightforward “do-it-yourself” kits for generating cell line and animal models in your lab. These kits are manufactured in our ISO:9001 quality management system certified facility in the USA using very optimized protocols and stringent quality control criteria.

The CRISPRCLEAR™ kits for cell line model generation are available as either custom designed kits for knock-in, knockout or point mutation models for a specific genomic locus, or as ready-to-use kits for transgene knock-in into a safe harbor locus, in any cell line, including iPSCs, blood-lineage cells and mouse cell lines. We use our proprietary designing strategy and next generation sequence validated gRNAs to ensure highly efficient gene modification in your cell line.

The CRISPRCRITTER™ kits are custom designed kits for generating mouse or rat models in an affordable and efficient manner in your own vivarium. The in vivo validated gRNAs provided in the kits ensure consistent and highly efficient gene modification in your final animal models.

Leverage our expertise in the CRISPR/Cas9 technology to generate a genetically modified cell line or animal model perfect for your research, in an affordable and convenient way.

  • Cell Line Model Generation

    The CRISPRCLEAR™ kits for cell line model generation are a do-it-yourself kits for affordable and efficient cell line model generation. A variety of kits are available for gene knockout, point mutation and knock-in models in human and mouse cell lines.

  • Animal Model Generation

    In vivo-grade, injection-ready CRISPR kits for the generation of transgenic mice and rats. 

     

 

We also offer products and kits using our proprietary TARGATT™ technology for efficient, site-specific gene insertion. The TARGATT™ products are an ideal toolkit for efficient knock-in of large transgenes which are usually not very efficient using CRISPR/Cas9.

 

SCHEMATIC-CRISPR


Choose the right gene editing technology

Applied StemCell also uses a propriety site-specific integrase-based gene editing technology, TARGATT™ for large fragment (up to 20 kb) knock-in in safe harbor locus. The complementary TARGATT™ and CRISPR/Cas9 technologies can be used together to generate advanced cell line and animal models very efficiently and effectively.

 

Project Purpose

CRISPR/Cas9

TARGATT™

Knock-Out (KO)

Yes

 

Point Mutation

Yes

 

Conditional KO

Yes

 

Knock-In

(<200 Nucleotide ssODN Donor)

Yes

 

Knock-In Transgenes in

Safe Harbor Loci (>2kb)

Challenging

(but limitations on size)

Yes

 (up to 20kb)

Knock-In

 (Plasmid DNA)

Challenging

(but limitations on size)

Yes

 (2 steps: KI docking site; KI transgene)