Human Fibroblast Immortalization Kit
Catalog # :
1 kit (5 x 20 μl)
It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells. Provided with this kit is the Virus Transduction Enhancer (ASK-3501-2) which is a mixture of polymers optimized for the infection of lentivirus or retrovirus in most cells. It can increase transduction rate up to 10 fold.