iPS Cells (iPSC from Fibroblasts, Episomal, Female, Control Lines for Genome Editing Services) *Academic Price

SKU :
ASE-9209
Catalog # :
ASE-9209
Size :
0.5 x 10^6 cells/vial
$1,260.00
Overview

MTA required.

Applied StemCell, Inc. provides Control Human Induced Pluripotent Stem (iPS) cells at low passages (p16). These pluripotent cells were generated from normal human skin fibroblasts using episomal reprogramming methods.  This method allows the transient expression of human transcription factors (OCT4, SOX2, KLF4, and c-MYC) that initiate the reprogramming process. The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for the expression of the pluripotency markers, including OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81, and alkaline phosphatase (AP) activity (Figure 1) and normal female karyotype (Figure 2). The ASE-9209 control human iPSC line can be used for CRISPR/Cas9 genome editing and differentiation to somatic lineages in vitro. Detailed protocols for thawing, culturing under both feeder and feeder-free conditions, and cryopreservation of these iPS cells are provided.

For donor information, please refer to datasheet or contact us.

Karyotype Analysis

Webpage-ASE-9209-karyotype

Karyotype analysis to rule out genetic aberrations. Cytogenic analysis was performed on G-banded metaphase cells from human iPSC line, ASE-9209. This iPSC line demonstrates a normal female karyotype.

Pluripotency Marker Analysis

webpage-ASE-9209-IHC-marker

Expression of pluripotency markers. ASC 9209 iPS cell line expresses common iPSC biomarkers (OCT4, SOX2, SSEA4, TRA-1-60,  and  TRA-1-81).  The corresponding DAPI staining is below each  marker staining image.  All images were taken at 10x magnification.

Alkaline Phosphatase (AP) staining

webpage-ASE-9209-morphology-AP

 ASC-9209 iPSCs stain positive for Alkaline Phosphatase: (a) A typical unstained colony (a) was used to gauge the extent of the AP staining (b). Both images were taken at 5x magnification.